Transcriptomics

Dataset Information

3

Overexpression of the lung cancer-prognostic miR-146b microRNAs has a minimal and negative effect on the malignant phenotype of A549 lung cancer cells


ABSTRACT: Biological effects of overexpression of miR-146b microRNAs in the A549 human lung cancer cell-line was studied. A549 cells were engineered to express the precursor RNA (pre-miR-146b) that generates the miR-146b microRNAs. Control cells were engineered using the same gene expression plasmid (pLemiR, Open Biosystems®) but without the pre-miR-146b insert. The Trans-Lentiviral GIPZ™ packaging system (Open Biosystems®) was used to generate stable transfectant populations of the engineered cells. Gene expression was compared between A549 stable transfectants of empty pLemiR plasmid (A549/vec cells) or of the pLemiR plasmid containing an insert for overexpressing human pre-miR-146b (A549/146b cells). Two different cell cultures were used for each cell-line (for biological replicates).

ORGANISM(S): Homo sapiens  

SUBMITTER: Santosh K Patnaik   Eric Kannisto  Santosh Kumar Patnaik  Sai Yendamuri  Reema Mallick 

PROVIDER: E-GEOD-29496 | ArrayExpress | 2011-05-24

SECONDARY ACCESSION(S): GSE29496PRJNA141661

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2012-08-31 | E-GEOD-40507 | ArrayExpress
2015-12-17 | E-GEOD-76050 | ArrayExpress
2013-05-11 | E-GEOD-46834 | ArrayExpress
2012-09-10 | E-GEOD-40708 | ArrayExpress
| GSE111493 | GEO
2013-09-16 | E-GEOD-45209 | ArrayExpress
2014-03-14 | E-GEOD-30131 | ArrayExpress
| GSE63511 | GEO
2007-09-01 | E-GEOD-3467 | ArrayExpress
2015-08-24 | E-GEOD-63511 | ArrayExpress