Transcriptomics

Dataset Information

239

Evidence-based annotation of genes and transcripts in Desulfovibrio vulgaris Hildenborough (RNA-Seq)


ABSTRACT: 5' RNASeq of mRNA from DvH in defined lactate/sulfate media (LS4D) One lane of sequence each for two different protocols applied to independently grown cells under similar conditions

ORGANISM(S): Desulfovibrio vulgaris str. Hildenborough  

SUBMITTER: Jennifer V Kuehl   Adam P Arkin  Adam M Deutschbauer  Morgan N Price 

PROVIDER: E-GEOD-29559 | ArrayExpress | 2011-05-27

SECONDARY ACCESSION(S): GSE29559SRP006901PRJNA154015

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Evidence-based annotation of transcripts and proteins in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough.

Price Morgan N MN   Deutschbauer Adam M AM   Kuehl Jennifer V JV   Liu Haichuan H   Witkowska H Ewa HE   Arkin Adam P AP  

Journal of bacteriology 20110812 20


We used high-resolution tiling microarrays and 5' RNA sequencing to identify transcripts in Desulfovibrio vulgaris Hildenborough, a model sulfate-reducing bacterium. We identified the first nucleotide position for 1,124 transcripts, including 54 proteins with leaderless transcripts and another 72 genes for which a major transcript initiates within the upstream protein-coding gene, which confounds measurements of the upstream gene's expression. Sequence analysis of these promoters showed that D.  ...[more]

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