Dataset Information


Circadian Clock Activity in Mouse and Human CD4+ T Cells

ABSTRACT: Though it is well established that immunological functions of CD4+ T cells are time of day-dependent, the underlying molecular mechanisms remain largely obscure. To address the question whether T cells themselves harbor a functional clock driving circadian rhythms of immune function, we analyzed clock gene expression and immune responses of CD4+ T cells purified from blood of healthy subjects at different time points throughout the day. Circadian clock function as well as immune function was further analyzed in cultivated T cells and circadian clock reporter systems. We found robust rhythms of clock gene expression as well as, after stimulation, of IFN-g production and CD40L expression in both freshly isolated and in cultured CD4+ T cells. Moreover, circadian luciferase reporter activities in CD4+ T cells and in thymic sections from PER2::LUCIFERASE reporter mice suggest that endogenous T cell clock rhythms are self-sustained under constant culture conditions. Microarray analysis of stimulated CD4+ T cell cultures revealed a rhythmic regulation of the NF-kB pathway as a candidate mechanism regulating circadian immune responses. Collectively, these data demonstrate for the first time that CD4+ T cell responses are regulated by an intrinsic cellular circadian oscillator capable of driving rhythmic adaptive immune responses in vitro and in vivo. The study is designed with 3 biological replicates from three different time points.

ORGANISM(S): Homo sapiens  

SUBMITTER: Juergen Westermann   Werner Solbach  Alexei Leliavski  Ludmila Skrum  Anton Leutz  Luigi Bonacina  Lennart Opitz  Tanja Lange  Henrik Oster  Thomas Bollinger  Christian Benedict  Judit Kovac 

PROVIDER: E-GEOD-29583 | ArrayExpress | 2012-04-30



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