Ontology highlight
ABSTRACT:
ORGANISM(S): Mycolicibacterium smegmatis
SUBMITTER: Gregory M Cook Jennifer R Robson Jennifer Robson Vickery L Arcus Joanna L McKenzie
PROVIDER: E-GEOD-29631 | ArrayExpress | 2012-06-14
SECONDARY ACCESSION(S): GSE29631PRJNA141261
REPOSITORIES: GEO, ArrayExpress
Journal of Bacteriology 20120224 9
The largest family of toxin-antitoxin (TA) modules are encoded by the vapBC operons, but their roles in bacterial physiology remain enigmatic. Microarray analysis in Mycobacterium smegmatis overexpressing VapC/VapBC revealed a high percentage of downregulated genes with annotated roles in carbon transport and metabolism, suggesting that VapC was targeting specific metabolic mRNA transcripts. To validate this hypothesis, purified VapC was used to identify the RNA cleavage site in vitro. VapC had ...[more]