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M. smegmatis vapBC::aphA-3 ΔvapBC mutant (JR121) harboring tetracycline inducible vector pMind containing the vapBC operon (pJR230) and the pMind vector containing the vapC toxin gene (pJR29)

ABSTRACT: Transcriptional profiling of M. smegmatis JR121 expressing VapC and VapBC grown in flasks on Hartmans de Bont medium supplemented with 0.2% glycerol Comparing transcriptional response of strain JR121 to conditional expression of VapC toxin compared with the expression of VapBC complex. Biological replicates: 4 independently grown and harvested. One replicate per array.

ORGANISM(S): Mycolicibacterium smegmatis

SUBMITTER: Gregory M Cook Jennifer R Robson Jennifer Robson Vickery L Arcus Joanna L McKenzie

PROVIDER: E-GEOD-29631 | ArrayExpress | 2012-06-14

SECONDARY ACCESSION(S): GSE29631PRJNA141261

REPOSITORIES: GEO, ArrayExpress

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A VapBC toxin-antitoxin module is a posttranscriptional regulator of metabolic flux in mycobacteria.

McKenzie Joanna L JL Robson Jennifer J Berney Michael M Smith Tony C TC Ruthe Alaine A Gardner Paul P PP Arcus Vickery L VL Cook Gregory M GM

Journal of Bacteriology 20120224 9

The largest family of toxin-antitoxin (TA) modules are encoded by the vapBC operons, but their roles in bacterial physiology remain enigmatic. Microarray analysis in Mycobacterium smegmatis overexpressing VapC/VapBC revealed a high percentage of downregulated genes with annotated roles in carbon transport and metabolism, suggesting that VapC was targeting specific metabolic mRNA transcripts. To validate this hypothesis, purified VapC was used to identify the RNA cleavage site in vitro. VapC had ...[more]

PMID: 22366418

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