Transcriptomics

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Exosomes isolated from M.tb infected cells modulate macrophage response to IFN-gamma


ABSTRACT: we studied the role of exosomes isolated from M.tb infected macrophages in modulating the macrophage response to IFN-γ. Nimblegen microarray gene expression studies were used to compare the suppression of IFN-γ inducible genes by exosomes relative to the virulent strain of M.tuberculosis. Overall our study suggest that exosomes, as carriers of M.tb pathogen associated molecular patterns (PAMPs), may provide a mechanistic link by which M.tb may exert its suppression of host immune response beyond the infected cell, and implies a physiological role for exosomes in immune surveillance of TB. Macrophages were treated with exosomes, infected with M.tb H37Rv or left untreated for 18 hours followed by +/- IFN-γ for an additional 18 hours. Cells were harvested and RNA was isolated and converted to double stranded cDNA and subsequently labeled and hybridized onto Mus musculus 4×72 Nimblegen microarray using Nimblegen Hybridization system 4 according to manufacturer’s instructions (Roche)

ORGANISM(S): Mus musculus  

SUBMITTER: Erliang Zeng  Prachi P Singh   John Tan   Christopher LeMaire   Jeffery S Schorey    

PROVIDER: E-GEOD-29731 | ArrayExpress | 2011-06-05

SECONDARY ACCESSION(S): GSE29731PRJNA141149

REPOSITORIES: GEO, ArrayExpress

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Publications

Exosomes released from M. tuberculosis infected cells can suppress IFN-γ mediated activation of naïve macrophages.

Singh Prachi P PP   LeMaire Christopher C   Tan John C JC   Zeng Erliang E   Schorey Jeffery S JS  

PloS one 20110414 4


Macrophages infected with Mycobacterium tuberculosis (M.tb) are known to be refractory to IFN-γ stimulation. Previous studies have shown that M.tb express components such as the 19-kDa lipoprotein and peptidoglycan that can bind to macrophage receptors including the Toll-like receptor 2 resulting in the loss in IFN-γ responsiveness. However, it is unclear whether this effect is limited to infected macrophages. We have previously shown that M.tb-infected macrophages release exosomes which are 30-  ...[more]

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