Transcriptomics

Dataset Information

8

Translational control in early adipogenesis


ABSTRACT: In obesity, adipose tissue expands by increasing the volume of existing adipocytes (hypertrophy), increasing the number of small adipocytes (hyperplasia) or both. Much is known about transcriptional control during adipogenesis, including early events. However, translational control plays a pivotal role in many dynamic processes and we presume that it is also important for the control of adipogenesis, especially in the first hours after hormonal induction. By use of ribosome profiling we identified 43 genes that are up-regulated and 2 genes that are down-regulated during the first six hours of adipogenesis in 3T3-L1 cells. Interestingly, we found Ghrelin to be down-regulated. Up-regulated genes comprise factors that are nucleic acid binding (Cdkn1c, eIF4b, Hsf1, Irf6, Myc, Plekhn1, Polr2a, Rpl18, Rpl27a, Rpl6, Rpl7a, Rps18, Rpsa, Sema3g, Tbc1d22a, Tsc22d3), form part of ribosomes (L6, L7a, L18, L27a, Sa, S18, S15a pseudogene), act on the regulation of translation (eIF4b) or transcription (Hsf1, Irf6, Myc, Tsc22d3). Others act as chaperones (Bag3, Hspa8/Hsc70, Hsp90ab1) or in other metabolic or signals transducing processes. We conclude that a moderate reorganisation of the functionality of the ribosomal machinery is a very important step for the growth and expression control at the beginning of adipogenesis. 12 samples: 4 (0h, non-polysomal fraction; 0h, polysomal fraction; 6h, non-polysomal fraction; 6h, polysomal fraction) x3 replicates

ORGANISM(S): Mus musculus  

SUBMITTER: Silvia von der Heyde   Bertram Brenig  Bernhard G Baumgartner  Carolin Fromm-Dornieden  Lennart Opitz  Olexandr Lytovchenko  Tim Beissbarth 

PROVIDER: E-GEOD-29744 | ArrayExpress | 2012-03-28

SECONDARY ACCESSION(S): GSE29744PRJNA141175

REPOSITORIES: GEO, ArrayExpress

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Publications

Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells.

Fromm-Dornieden Carolin C   von der Heyde Silvia S   Lytovchenko Oleksandr O   Salinas-Riester Gabriela G   Brenig Bertram B   Beissbarth Tim T   Baumgartner Bernhard G BG  

BMC molecular biology 20120321


BACKGROUND: Control of translation allows for rapid adaptation of the cell to stimuli, rather than the slower transcriptional control. We presume that translational control is an essential process in the control of adipogenesis, especially in the first hours after hormonal stimulation. 3T3-L1 preadipocytes were cultured to confluency and adipogenesis was induced by standard protocols using a hormonal cocktail. Cells were harvested before and 6 hours after hormonal induction. mRNAs attached to ri  ...[more]

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