Transcriptomics

Dataset Information

7

An environment with strong gravitational and magnetic field alterations synergizes to promote variations in Arabidopsis thaliana callus global transcriptional state


ABSTRACT: Using diamagnetic levitation, we have exposed A. thaliana in vitro callus cultures to five environments with different levels of effective gravity (from levitation i.e. simulated mg* to 2g*) and magnetic fields (10.1 to 16.5 Tesla) and we have compared the results with those of similar experiments done in a Random Position Machine (simulated micro g) and a Large Diameter Centrifuge (2g) free of high magnetic fields. Microarray analysis indicates that there are changes in overall gene expression of the cultured cells exposed to these unusual environments but also that gravitational and magnetic field produce synergic variations in the steady state of the transcriptional profile of A. thaliana. Significant changes in the expression of structural, abiotic stress and secondary metabolism genes were observed into the magnet field. These results confirm that the strong magnetic field, both at micro g* or 2g*, has a significant effect on the expression of these genes but subtle gravitational effects are still observable. These subtle responses to microgravity environments are opposite to the ones observed in a hypergravity one. seven-condition experiment, MM2D Arabidopsis culture callus control vs. Treatment (altered gravity simulation, GBF). Three GBF were used (LDC (2g) + control, RPM (mg) + control and Magnet (mg*, 0.1g*, 1g*, 1.9g*, 2g*) + control). Biological replicates: 3 replicates in all conditions and controls except 1.9g* (2 replicates)

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Raúl Herranz   Jack J van Loon  F J Medina  Juana M Gonzalez-Rubio  Ana I Manzano  Peter Christianen  Raul Herranz 

PROVIDER: E-GEOD-29787 | ArrayExpress | 2012-01-01

SECONDARY ACCESSION(S): GSE29787PRJNA141065

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Spaceflight engages heat shock protein and other molecular chaperone genes in tissue culture cells of Arabidopsis thaliana.

Zupanska Agata K AK   Denison Fiona C FC   Ferl Robert J RJ   Paul Anna-Lisa AL  

American journal of botany 20121220 1


PREMISE OF THE STUDY: Gravity has been a major force throughout the evolution of terrestrial organisms, and plants have developed exquisitely sensitive, regulated tropisms and growth patterns that are based on the gravity vector. The nullified gravity during spaceflight allows direct assessment of gravity roles. The microgravity environments provided by the Space Shuttle and International Space Station have made it possible to seek novel insights into gravity perception at the organismal, tissue  ...[more]

Similar Datasets

2012-01-01 | GSE29787 | GEO
2015-02-02 | E-GEOD-62128 | ArrayExpress
2013-06-26 | E-GEOD-33801 | ArrayExpress
| GSE33779 | GEO
2016-08-24 | E-GEOD-85968 | ArrayExpress
2017-04-24 | E-MTAB-4698 | ArrayExpress
2019-07-09 | E-MTAB-8064 | ArrayExpress
2010-07-01 | E-MEXP-2082 | ArrayExpress
2015-02-27 | E-MTAB-3290 | ArrayExpress
| GSE80323 | GEO