Transcriptomics

Dataset Information

3

Tuberous sclerosis complex 1 (Tsc1) enforces quiescence of naive T cells to promote immune homeostasis and function


ABSTRACT: The mechanisms that regulate T cell quiescence are poorly understood. We report that tuberous sclerosis complex 1 (Tsc1) establishes a quiescent program in naïve T cells by controlling cell size, cell cycle entry, and responses to T cell receptor stimulation. Loss of quiescence predisposed Tsc1-deficient T cells to apoptosis that depleted conventional T cells and invariant natural killer T cells. Loss of Tsc1 function dampened in vivo immune responses to bacterial infection. Tsc1-deficient T cells exhibited increased mTORC1 but diminished mTORC2 activities, with mTORC1 activation essential for the disruption of immune homeostasis. Therefore, Tsc1-dependent control of mTOR is crucial in establishing naïve T cell quiescence to facilitate adaptive immune function. Naïve CD4 and CD8 T cells from wild-type and Tsc1-deficient mice (in triplicates each group) were stimulated with or without TCR signaling. RNA was analyzed by microarrays. WT/KO for 0 and 4 hr for CD4 (triplicates), and WT/KO for 0 hr for CD8 (duplicates).

ORGANISM(S): Mus musculus  

SUBMITTER: D R Green   G Neale  H Chi  David Finkelstein  K Yang  W He 

PROVIDER: E-GEOD-29797 | ArrayExpress | 2011-07-11

SECONDARY ACCESSION(S): GSE29797PRJNA141013

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2014-01-27 | E-GEOD-51668 | ArrayExpress
2017-03-29 | PXD005492 | Pride
2017-03-15 | PXD004367 | Pride
2019-03-28 | PXD012058 | Pride
2015-01-20 | E-GEOD-59846 | ArrayExpress
2016-04-22 | E-GEOD-59846 | ExpressionAtlas
2011-12-01 | E-GEOD-30196 | ArrayExpress
2014-08-15 | E-GEOD-57846 | ArrayExpress
2009-03-28 | E-GEOD-15324 | ArrayExpress
2015-01-20 | E-GEOD-58684 | ArrayExpress