Transcriptomics

Dataset Information

4

Cold hardening gene expression


ABSTRACT: Cold hardening treatment, a brief exposure to low temperatures (e.g. 0°C for 2 h), can protect certain insects against subsequent exposure to temperatures sufficiently low to cause damage or lethality. Microarray analysis to examine the changes in transcript abundance associated with cold hardening has been undertaken in Drosophila melanogaster in order to gain insight into this phenomenon. Transcripts associated with 36 genes were identified, a subset of which appeared to be also differentially expressed after heat shock treatment. Quantitative RT-PCR was used to independently determine transcript abundance of a subset of these sequences. Taken together, these assays suggest that stress proteins, including Hsp23, Hsp26, Hsp83 and Frost as well as membrane-associated proteins may contribute to the cold hardening response. Co-reared flies were separated into a control group and a treatment group at random. RNA was isolated from the cold-shocked flies and the respective controls and was used for direct comparisons on cDNA microarrays. Treatments were not varied as they had been optimized in previous studies. A pilot experiment was performed using the Drosophila 7k2 array (GPL311) and subsequently three pairs of independent biological samples were evaluated with the Drosophila 12k1 array (GPL1467).

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Virginia K Walker   J T Westwood  R M Robertson  Wensheng Qin  Scott J Neal 

PROVIDER: E-GEOD-2989 | ArrayExpress | 2005-11-30

SECONDARY ACCESSION(S): GSE2989PRJNA91941

REPOSITORIES: GEO, ArrayExpress

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Publications

Cold hardening and transcriptional change in Drosophila melanogaster.

Qin W   Neal SJ   Robertson RM   Westwood JT   Walker VK  

Insect molecular biology 20051201 6


Cold hardening treatment - a brief exposure to low temperatures - can protect certain insects against subsequent exposure to temperatures sufficiently low to cause damage or lethality. Microarray analysis to examine the changes in transcript abundance associated with cold hardening treatment (0 degrees C for 2 h followed by 30 min recovery at 25 degrees C) was undertaken in Drosophila melanogaster in order to gain insight into this phenomenon. Transcripts associated with 36 genes were identified  ...[more]

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