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Expression data from CD4 single positive thymocyte subsets from C57BL/6 mice of 6-8 wks of age

ABSTRACT: After positive selection in the thymus, the newly generated single positive (SP) thymocytes are phenotypically and functionally immature and undergo apoptosis upon antigen stimulation. In the thymic medullary microenvironment, SP cells progressively acquire immunocompetence. Negative selection to remove autoreactive T cells also occur at this stage. We have defined four subsets of CD4 SP, namely, SP1, SP2, SP3, and SP4 that follow a functional maturation program and a sequential emergence during mouse ontogeny. We used microarray to detail the global programm of gene expression during the maturation of murine CD4 single positive thymocytes Four subsets of CD4+CD8-CD25-NK1.1- thymocytes from C57BL/6 mice of 6-8 wks of age were purified for RNA extraction and hybridization on Affymetrix microarrays, namely, SP1 (Qa-2-6C10+CD69+), SP2 (Qa-2-6C10-CD69+), SP3 (Qa-2-6C10-CD69-), SP4 (Qa-2+6C10-CD69-).

ORGANISM(S): Mus musculus  

SUBMITTER: Wei-Feng Chen   Yu Zhang  Yubin Zhou  Qing Ge  Fei Teng 

PROVIDER: E-GEOD-30083 | ArrayExpress | 2012-02-13



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The molecular signature underlying the thymic migration and maturation of TCRαβ+ CD4+ CD8 thymocytes.

Teng Fei F   Zhou Yubin Y   Jin Rong R   Chen Yu Y   Pei Xiaoyan X   Liu Yuanfeng Y   Dong Jie J   Wang Wei W   Pang Xuewen X   Qian Xiaoping X   Chen Wei-Feng WF   Zhang Yu Y   Ge Qing Q  

PloS one 20111013 10

BACKGROUND: After positive selection, the newly generated single positive (SP) thymocytes migrate to the thymic medulla, where they undergo negative selection to eliminate autoreactive T cells and functional maturation to acquire immune competence and egress capability. METHODOLOGY/PRINCIPAL FINDINGS: To elucidate the genetic program underlying this process, we analyzed changes in gene expression in four subsets of mouse TCRαβ(+)CD4(+)CD8(-) thymocytes (SP1 to SP4) representative of sequential s  ...[more]

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