Transcriptomics,Multiomics

Dataset Information

34

Transcription profiling by array of Arabidopsis root cells after sulphur deprivation


ABSTRACT: Cell-type specific transcriptional profiles were generated by FACS (Fluorescence Activated Cell Sorting) sorting of roots that express cell-type specific GFP-reporters. Five different GFP-reporter lines were used. FACS cell populations were isolated from roots grown under sulfur deficient conditions for 3 hours. Stress responses in plants are tightly coordinated with developmental processes, but the interaction between these pathways is poorly understood. Here we use genome-wide assays at high spatial and temporal resolution to understand the processes that lnk development and stress in the Arabidopsis root. Our meta-analysis finds little evidence for a universal stress response. Common stress responses appear to exists and, analagous to animal systems, many of them show cell-type specificity, suggesting a convergent evolutionary theme in multicellular organisms. Common stress responses may be mediated by cell identity regulators, as mutations in these genes resulted in altered responses to stress. Our results reveal surprising linkages between stress and development at cellular resolution, and show the power of multiple genome-wide datasets to elucidate biological processes. 3 replicates for each of 5 cell types for sulfur deficient media

OTHER RELATED OMICS DATASETS IN: E-GEOD-30095

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Anjali S Iyer-Pascuzzi  Philip N Benfey    

PROVIDER: E-GEOD-30099 | ArrayExpress | 2014-08-22

SECONDARY ACCESSION(S): GSE30099PRJNA155181

REPOSITORIES: GEO, ArrayExpress

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Publications

Cell identity regulators link development and stress responses in the Arabidopsis root.

Iyer-Pascuzzi Anjali S AS   Jackson Terry T   Cui Hongchang H   Petricka Jalean J JJ   Busch Wolfgang W   Tsukagoshi Hironaka H   Benfey Philip N PN  

Developmental cell 20111001 4


Stress responses in plants are tightly coordinated with developmental processes, but interaction of these pathways is poorly understood. We used genome-wide assays at high spatiotemporal resolution to understand the processes that link development and stress in the Arabidopsis root. Our meta-analysis finds little evidence for a universal stress response. However, common stress responses appear to exist with many showing cell type specificity. Common stress responses may be mediated by cell ident  ...[more]

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