Transcriptomics

Dataset Information

8

Genome-wide espression kinetics of children progressing to clinical Type 1 diabetes (T1D).


ABSTRACT: To unravel genes and molecular pathways involved in the pathogenesis of type 1 diabetes (T1D), we performed genome-wide gene expression profiling of prospective venous blood samples from children developing T1D-associated autoantibodies or progressing towards clinical diagnosis. 247 peripheral blood RNA samples from 18 prediabetic children and their matched controls were analyzed with Illumina Human HT-12 Expression BeadChips version 3 arrays, in order to study the gene expression changes occuring during the pathogenesis of Type 1 diabetes (T1D). Each case child (with T1D-specific autoantibodies) was matched with a persistently autoantibody-negative control child, with the same HLA-DQB1 risk category, gender, and place and date of birth. Two control children were selected for T1D cases 3, 5, 13 and 17. Seroconversion is determined as the first detection of T1D-specific autoantibody/autoantibodies (ICA titre >4 JDFU, IAA >3.47 RU, GADA >5.4 RU, IA-2A >0.43 RU, ZnT8A >0.61 RU).

ORGANISM(S): Homo sapiens  

SUBMITTER: Harri Lähdesmäki   Cisca Wijmenga  Heikki Hyöty  Jorma Ilonen  Essi Laajala  Riitta Veijola  Mikael Knip  Riitta Lahesmaa  Tuula Simell  Juha Mykkänen  Matti Vaarma  Isis Ricaño-Ponce  Laura L Elo  Olli Simell  Teemu D Laajala  Henna Kallionpää 

PROVIDER: E-GEOD-30210 | ArrayExpress | 2014-02-20

SECONDARY ACCESSION(S): GSE30210PRJNA155103

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications


The insult leading to autoantibody development in children who will progress to develop type 1 diabetes (T1D) has remained elusive. To investigate the genes and molecular pathways in the pathogenesis of this disease, we performed genome-wide transcriptomics analysis on a unique series of prospective whole-blood RNA samples from at-risk children collected in the Finnish Type 1 Diabetes Prediction and Prevention study. We studied 28 autoantibody-positive children, out of which 22 progressed to cli  ...[more]

Similar Datasets

2014-02-20 | E-GEOD-30209 | ArrayExpress
2014-02-20 | E-GEOD-30208 | ArrayExpress
2014-02-20 | E-GEOD-43488 | ArrayExpress
| GSE43488 | GEO
2010-03-31 | E-TABM-666 | ArrayExpress
2015-11-02 | E-TABM-666 | ExpressionAtlas
2018-07-16 | MTBLS620 | MetaboLights
2014-02-20 | E-GEOD-30211 | ArrayExpress
2014-04-10 | E-GEOD-51058 | ArrayExpress
2018-11-07 | PXD008870 | Pride