Transcriptomics

Dataset Information

3

Transcription profiling of SKOV3 ovarian carcinoma cells overexpressing BRCA1


ABSTRACT: Follistatin is a folliculogenesis regulating protein that has been found in relatively high concentration in the female ovarian tissues. Follistatin acts as an antagonist to the function of Activin, which is often found elevated in ovarian carcinogenesis and thus presents a possibility for therapeutic intervention in controlling ovarian cancer. Most of the ovarian cancer occurs in its ovarian surface epithelium (OSE) cells. Although breast cancer susceptibility gene 1 (BRCA1) is a known tumor suppressor for breast cancer but its role in ovarian cancer is beginning to unfold. We have shown that in ovarian carcinoma cells (SKOV3), stable overexpression of BRCA1 stimulates Follistatin secretion and simultaneously downregulates Activin expression. Moreover, knock down of BRCA1 in immortalized OSE (IOSE) cells from human ovarian tissue demonstrates downregulation of Follistatin secretion with simultaneous up regulation of Activin expression. IOSE cells generated from an ovarian cancer patient with BRCA1 mutation failed to secrete Follistatin in the medium. Our results indicate a novel function for BRCA1 in the form of regulation of the expression of Follistatin in the ovarian cells. 3 treatments vs 3 controls

REANALYSED by: E-GEOD-30296

ORGANISM(S): Homo sapiens  

SUBMITTER: Anju Preet   Eliot M Rosen  Rosie Sneed  Jingwen Xu  Clara Salamanca  Tejaswita Karve  Tapas Saha  Xin James Li  Deepak Kumar 

PROVIDER: E-GEOD-30296 | ArrayExpress | 2011-07-02

SECONDARY ACCESSION(S): GSE30296PRJNA143795

REPOSITORIES: GEO, ArrayExpress

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Publications

BRCA1 regulates follistatin function in ovarian cancer and human ovarian surface epithelial cells.

Karve Tejaswita M TM   Preet Anju A   Sneed Rosie R   Salamanca Clara C   Li Xin X   Xu Jingwen J   Kumar Deepak D   Rosen Eliot M EM   Saha Tapas T  

PloS one 20120601 6


Follistatin (FST), a folliculogenesis regulating protein, is found in relatively high concentrations in female ovarian tissues. FST acts as an antagonist to Activin, which is often elevated in human ovarian carcinoma, and thus may serve as a potential target for therapeutic intervention against ovarian cancer. The breast cancer susceptibility gene 1 (BRCA1) is a known tumor suppressor gene in human breast cancer; however its role in ovarian cancer is not well understood. We performed microarray  ...[more]

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