Transcriptomics

Dataset Information

4

The ets transcription factor ELF5 suppresses the estrogen sensitive phenotype and contributes to antiestrogen resistance in luminal breast cancer. [human]


ABSTRACT: The ets transcription factor ELF5 specifies the differentiation of mammary progenitor cells to establish the milk-secreting lineage. ER- and poor prognosis basal breast cancers arise from this progenitor cell and these cancers express high levels of Elf5. Knockdown of ELF5 expression in basal breast cancer cell lines, or forced expression in luminal breast cancer cell lines, resulted in reduced cell proliferation. Transcript profiling and chromatin immunoprecipitation revealed that the transcriptional activity of ELF5 specified the gene expression patterns that distinguish basal from luminal breast cancer, including suppression of FOXA1, GATA3 and ER, key estrogen-action genes. Tamoxifen treatment of luminal MCF7 cells upregulated Elf5 expression and cells that acquired resistance to Tamoxifen became dependent on ELF5 for proliferation. ELF5 is a regulator of breast cancer cell proliferation, transcriptionally specifies the basal molecular subtype and is utilised by ER+ breast cancer cells to escape proliferative arrest caused by Tamoxifen. Elf5 was knocked down via siRNA in basal HCC1937 cell lines, in triplicate. Elf5 was induced in luminal T47D and MCF7 cell lines via a doxycycline inducible expression vector, in duplicate.

ORGANISM(S): Homo sapiens  

SUBMITTER: Alex Swarbrick   Stephanie Allerdice  Cara Evans  Maria Kalyuga  Chehani Alles  David Gallego-Ortega  Tilman Brummer  Robert I Nicholson  Mark Cowley  Liz Caldon  Rosalind Launchbury  Matthew J Naylor  Adelaide Young  Andrea Egger  Susan Clark  Fatima Valdes-Mora  Nicola Armstrong  Aaron Statham  Catherine Piggin  Julia M Gee  Mark J Cowley  Robert L Sutherland  Heather Lee  Wendy Au  Jason Carroll  Christopher J Ormandy  Andrew Stone  Warren Kaplan 

PROVIDER: E-GEOD-30405 | ArrayExpress | 2012-12-27

SECONDARY ACCESSION(S): GSE30405PRJNA154827

REPOSITORIES: GEO, ArrayExpress

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