Genomics

Dataset Information

298

Dual Role of FoxA1 in Androgen Receptor Binding to Chromatin, Androgen Signaling and Prostate Cancer [ChIP_seq, DHS_seq]


ABSTRACT: We report the dual role of FoxA1 in androgen receptor recruitment to the chromatin of androgen responsive prostate cancer cell line LNCaP-1F5 using ChIP-sequencing. Depletion of FoxA1 reprograms both androgen and glucocorticoid receptor recruitment and subsequent gene expression. The ChIP-seq has been performed using AR, FoxA1, GR, H3K4me2 antibodies. We have also mapped the DNaseI-hypersensitive sites (DHS) using deep sequencing. Examination of AR, FoxA1, GR, H3K4me2 binding sites and DHS sites in parental and FoxA1 depleted LNCaP-1F5 cells.

ORGANISM(S): Homo sapiens  

SUBMITTER: Mikael Lundin   Juha-Pekka Turunen  Juho Konsti  Olli Kallioniemi  Olli A. Jänne  Stig Nordling  Sampsa Hautaniemi  Tiina Vesterinen  Olli A Jänne 

PROVIDER: E-GEOD-30623 | ArrayExpress | 2011-09-14

SECONDARY ACCESSION(S): SRP007486GSE30623PRJNA154785

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


High androgen receptor (AR) level in primary tumour predicts increased prostate cancer-specific mortality. However, the mechanisms that regulate AR function in prostate cancer are poorly known. We report here a new paradigm for the forkhead protein FoxA1 action in androgen signalling. Besides pioneering the AR pathway, FoxA1 depletion elicited extensive redistribution of AR-binding sites (ARBs) on LNCaP-1F5 cell chromatin that was commensurate with changes in androgen-dependent gene expression s  ...[more]

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