Genomics

Dataset Information

90

Genome-wide binding map of the HIV Tat protein to the human genome (ChIP-Seq)


ABSTRACT: The HIV-1 Trans-Activator of Transcription (Tat) protein binds to multiple host cellular factors and greatly enhances the level of transcription of the HIV genome. Here, we report the genome-wide binding map of Tat to the human genome in Jurkat T cells (Jurkat-Tat cells) using chromatin immunoprecipitation combined with next-generation sequencing. cDNA microarray was used to monitor gene expression changes between Jurkat and Jurkat-Tat cells. Additionally, we compared distribution of H3K9ac near gene promoters between Jurkat and Jurkat-Tat cells using ChIP-chip method and hybridized onto Agilent promoter array. Our data reveal that Tat’s interaction with the host genome is more extensive than previously thought, with potentially important implications for the viral life cycle. Expression profiles on Jurkat-Tat cells versus Jurkat cells. ChIP on chip for H3K9ac in Jurkat-Tat versus Jurkat cells. ChIP-seq for HIV-1 Tat protein in Jurkat-Tat cells.

ORGANISM(S): Homo sapiens  

SUBMITTER: Trent Su  Celine Marrban   Siavash K Kurdistani   Siavash Kurdistani    

PROVIDER: E-GEOD-30738 | ArrayExpress | 2013-04-03

SECONDARY ACCESSION(S): GSE30738SRP007528PRJNA154551

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Genome-wide binding map of the HIV-1 Tat protein to the human genome.

Marban Céline C   Su Trent T   Ferrari Roberto R   Li Bing B   Vatakis Dimitrios D   Pellegrini Matteo M   Zack Jerome A JA   Rohr Olivier O   Kurdistani Siavash K SK  

PloS one 20111104 11


The HIV-1 Trans-Activator of Transcription (Tat) protein binds to multiple host cellular factors and greatly enhances the level of transcription of the HIV genome. While Tat's control of viral transcription is well-studied, much less is known about the interaction of Tat with the human genome. Here, we report the genome-wide binding map of Tat to the human genome in Jurkat T cells using chromatin immunoprecipitation combined with next-generation sequencing. Surprisingly, we found that ~53% of th  ...[more]

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