Transcriptomics

Dataset Information

300

SuperSAGE evidence for CD14++CD16+ monocytes as a third monocyte subset


ABSTRACT: Monocytes are a heterogeneous cell population with subset-specific functions and phenotypes. The differential expression of CD14 and CD16 distinguishes classical CD14++CD16-, intermediate CD14++CD16+ and non-classical CD14+CD16++ monocytes. However, CD14++CD16+ monocytes remain the most poorly characterized subset so far. Therefore we analyzed the transcriptomes of the three monocyte subsets using SuperSAGE in combination with high-throughput sequencing. Analysis of 5,487,603 tags revealed unique identifiers of CD14++CD16+ monocytes, delineating these cells from the two other monocyte subsets. CD14++CD16+ monocytes were linked to antigen processing and presentation (e.g. CD74, HLA-DR, IFI30, CTSB), to inflammation and monocyte activation (e.g. TGFB1, AIF1, PTPN6), and to angiogenesis (e.g. TIE2, CD105). Therefore we provide genetic evidence for a distinct role of CD14++CD16+ monocytes in human immunity. Human monocyte subsets (CD14++CD16-, CD14++CD16+, CD14+CD16++) were isolated from 12 healthy volunteers based on MACS technology. Total RNA from monocyte subsets was isolated and same aliquots from each donor and monocyte subset were matched for SuperSAGE. Three SuperSAGE libraries (CD14++CD16-, CD14++CD16+ and CD14+CD16++) were generated.

ORGANISM(S): Homo sapiens  

SUBMITTER: Kyrill S Rogacev   Danilo Fliser  Björn Rotter  Gunnar H Heine  Peter Winter  Adam M Zawada  Rolf R Marell 

PROVIDER: E-GEOD-30811 | ArrayExpress | 2011-07-24

SECONDARY ACCESSION(S): SRP007569GSE30811PRJNA144215

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

SuperSAGE evidence for CD14++CD16+ monocytes as a third monocyte subset.

Zawada Adam M AM   Rogacev Kyrill S KS   Rotter Björn B   Winter Peter P   Marell Rolf-R RR   Fliser Danilo D   Heine Gunnar H GH  

Blood 20110729 12


Monocytes are a heterogeneous cell population with subset-specific functions and phenotypes. The differential expression of CD14 and CD16 distinguishes classical CD14(++)CD16(-), intermediate CD14(++)CD16(+), and nonclassical CD14(+)CD16(++) monocytes. Current knowledge on human monocyte heterogeneity is still incomplete: while it is increasingly acknowledged that CD14(++)CD16(+) monocytes are of outstanding significance in 2 global health issues, namely HIV-1 infection and atherosclerosis, CD14  ...[more]

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