Genomics

Dataset Information

296

Splicing enhances recruitment of methyltransferase HYPB/Setd2 and methylation of histone H3 lysine 36


ABSTRACT: Several lines of recent evidence support a role for chromatin in splicing regulation. Here we show that splicing can also contribute to histone modification, which implies a bidirectional communication between epigenetics and RNA processing. Genome-wide analysis of histone methylation in human cell lines and mouse primary T cells reveals that intron-containing genes are preferentially marked with H3K36me3 relative to intronless genes. In intron-containing genes, H3K36me3 marking is proportional to transcriptional activity, whereas in intronless genes H3K36me3 is always detected at much lower levels. Furthermore, splicing inhibition impairs recruitment of H3K36 methyltransferase HYPB/Setd2 and reduces H3K36me3, whereas splicing activation has the opposite effect. Moreover, the increase of H3K36me3 correlates with the length of the first intron, consistent with the view that splicing enhances H3 methylation. We propose that splicing is mechanistically coupled to recruitment of HYPB/Setd2 to elongating RNA Polymerase II. This experiment proposes to profile genome-wide binding profiles by ChIP-seq (Illumina, 36 bp tags) of RNA polymerase II (one biological replicate), the histone modification H3K36me3 (2 replicates) and a reference control input sample (genomic DNA after reverse cross-link, one replicate) in a human H1299 lung carcinoma cell line *** Raw data not provided for Samples GSM766322-GSM766324.

ORGANISM(S): Homo sapiens  

SUBMITTER: Maria Carmo-Fonseca   Jorge Andrade  Jean-Christophe Andrau  Ivo Gut  Dirk Eick  Helena Levezinho  Romain Fenouil  Pierre Ferrier  Silvia Carvalho  Frederic Koch  Sérgio de Almeida  Marta Gut  Ana Grosso 

PROVIDER: E-GEOD-30902 | ArrayExpress | 2011-07-25

SECONDARY ACCESSION(S): SRP007570GSE30902PRJNA144241

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Splicing enhances recruitment of methyltransferase HYPB/Setd2 and methylation of histone H3 Lys36.

de Almeida Sérgio Fernandes SF   Grosso Ana Rita AR   Koch Frederic F   Fenouil Romain R   Carvalho Sílvia S   Andrade Jorge J   Levezinho Helena H   Gut Marta M   Eick Dirk D   Gut Ivo I   Andrau Jean-Christophe JC   Ferrier Pierre P   Carmo-Fonseca Maria M  

Nature structural & molecular biology 20110726 9


Several lines of recent evidence support a role for chromatin in splicing regulation. Here, we show that splicing can also contribute to histone modification, which implies bidirectional communication between epigenetic mechanisms and RNA processing. Genome-wide analysis of histone methylation in human cell lines and mouse primary T cells reveals that intron-containing genes are preferentially marked with histone H3 Lys36 trimethylation (H3K36me3) relative to intronless genes. In intron-containi  ...[more]

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