Transcriptomics

Dataset Information

5

Rice gene global expression during nonhost interaction with Blumeria graminis f. sp. hordei (Bgh)


ABSTRACT: Powdery mildew is a very common plant disease and only few plants are immune. Host interactions have been identified and characterized for the pathosystems barley-B. graminis f. sp. tritici (Bgt) and wheat-B. graminis f. sp. hordei (Bgh), whereas no data are reported about powdery mildew and nonhost plants, such as rice. On the other hand rice nonhost resistance is widely unexploited and only few expression data are available. To characterize rice response during nonhost interaction with Bgh, a global expression analysis was performed by using the GeneChip® Rice Genome Array. To describe rice gene expression profiles during nonhost interaction, 2 week-old rice plantlets were inoculated with Bgh. Treated (inoculated) and control (mock) samples were collected 24 hours post-inoculation for GeneChip® Rice Genome Array hybridization. For transcript profiling experiments with powdery mildew, entire leaves were sampled from rice plantlets 2 weeks old (cv. Nipponbare). Treated and control (mock) rice leaves were collected 24 hours post inoculation. Three biological replicates for inoculated and control plants were extracted and analysed independently with the GeneChip® Rice Genome Array.

ORGANISM(S): Oryza sativa  

SUBMITTER: Pamela Abbruscato   PAMELA ABBRUSCATO  Odile Faivre-Rampant  Marcello Del Corvo 

PROVIDER: E-GEOD-30942 | ArrayExpress | 2012-03-26

SECONDARY ACCESSION(S): GSE30942PRJNA146299

REPOSITORIES: GEO, ArrayExpress

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Publications


With the aim of identifying novel regulators of host and nonhost resistance to fungi in rice, we carried out a systematic mutant screen of mutagenized lines. Two mutant wrky22 knockout lines revealed clear-cut enhanced susceptibility to both virulent and avirulent Magnaporthe oryzae strains and altered cellular responses to nonhost Magnaporthe grisea and Blumeria graminis fungi. In addition, the analysis of the pathogen responses of 24 overexpressor OsWRKY22 lines revealed enhanced resistance ph  ...[more]

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