Transcriptomics

Dataset Information

16

Primary and secondary CD8 T cells during acute and chronic LCMV infection


ABSTRACT: Understanding the response of memory CD8 T cells to persistent antigen re-stimulation and the role of CD4 T cell help is critical to the design of successful vaccines for chronic diseases. However, studies comparing the protective abilities and qualities of memory and naïve cells have been mostly performed in acute infections, and little is known about their roles during chronic infections. Herein, we show that memory cells dominate over naïve cells and are protective when present in large enough numbers to quickly reduce infection. In contrast, when infection is not rapidly reduced, memory cells are quickly lost, unlike naïve cells. This loss of memory cells is due to (i) an early block in cell proliferation, (ii) selective regulation by the inhibitory receptor 2B4, and (iii) increased reliance on CD4 T cell help. These findings have important implications towards the design of T cell vaccines against chronic infections and tumors. 16 samples are analyzed: 3 replicates of secondary effector CD8 P14 T cells at day 8 post-acute lymphocytic choriomeningitis virus (LCMV) infection; 4 replicates of secondary effector CD8 P14 T cells at day 8 post-chronic LCMV infection; 4 replicates of primary effector CD8 P14 T cells at day 8 post-acute LCMV infection; and 5 replicates of primary effector CD8 P14 T cells at day 8 post-chronic LCMV infection.

ORGANISM(S): Mus musculus  

SUBMITTER: W N Haining   Erin E West  Ben Youngblood  Wendy G Tan  Hyun-Tak Jin  Rafi Ahmed  Slivia Calpe  Cox Terhorst  Koichi Araki  Erin West  Bogumila T Konieczny  Gordon J Freeman  Gabriela Alexe 

PROVIDER: E-GEOD-30962 | ArrayExpress | 2011-08-22

SECONDARY ACCESSION(S): GSE30962PRJNA146161

REPOSITORIES: GEO, ArrayExpress

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Publications

Tight regulation of memory CD8(+) T cells limits their effectiveness during sustained high viral load.

West Erin E EE   Youngblood Ben B   Tan Wendy G WG   Jin Hyun-Tak HT   Araki Koichi K   Alexe Gabriela G   Konieczny Bogumila T BT   Calpe Silvia S   Freeman Gordon J GJ   Terhorst Cox C   Haining W Nicholas WN   Ahmed Rafi R  

Immunity 20110801 2


To design successful vaccines for chronic diseases, an understanding of memory CD8(+) T cell responses to persistent antigen restimulation is critical. However, most studies comparing memory and naive cell responses have been performed only in rapidly cleared acute infections. Herein, by comparing the responses of memory and naive CD8(+) T cells to acute and chronic lymphocytic choriomeningitis virus infection, we show that memory cells dominated over naive cells and were protective when present  ...[more]

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