Transcriptomics

Dataset Information

3

Expression analysis of ZBP-89 deficient human primary erythroid progenitors


ABSTRACT: The molecular mechanisms underlying erythroid-specific gene regulation remain incompletely understood. Closely spaced binding sites for GATA, NF-E2/maf and CACCC interacting transcription factors play functionally important roles in globin and other erythroid-specific gene expression. We and others recently identified the CACCC-binding transcription factor ZBP-89 as a novel GATA-1 and NF-E2/mafK interacting partner. Here, we examined the role of ZBP-89 in human globin gene regulation and erythroid maturation using a primary CD34+ cell ex vivo differentiation system. We show that ZBP-89 protein levels rise dramatically during human erythroid differentiation, and that ZBP-89 occupies key cis-regulatory elements within the globin and other erythroid gene loci. ZBP-89 binding correlates strongly with RNA Pol II occupancy, active histone marks, and high-level gene expression. ZBP-89 physically associates with the histone acetyltransferases (HATs) p300 and Gcn5/Trrap, and occupies common sites with Gcn5 within the human globin loci. Lentiviral shRNA knockdown of ZBP-89 results in reduced Gcn5 occupancy, decreased acetylated histone 3 levels, lower globin and erythroid-specific gene expression, and impaired erythroid maturation. Addition of the HDAC inhibitor valproic acid partially reverses the reduced globin gene expression. These findings reveal an activating role for ZBP-89 in human globin gene regulation and erythroid differentiation. Keywords: Human primary erythroid progenitors Expression data of human erythroid progenitors transduced with lentivirus expressing short hairpins against ZBP-89 and control empty vector

ORGANISM(S): Homo sapiens  

SUBMITTER: A J Woo   J Xu  A B Cantor  J Kim  H Huang  Andrew Jonghan Woo 

PROVIDER: E-GEOD-31092 | ArrayExpress | 2011-08-01

SECONDARY ACCESSION(S): GSE31092PRJNA144709

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2011-07-30 | GSE31036 | GEO
2011-10-06 | E-GEOD-26351 | ArrayExpress
2010-12-07 | E-GEOD-22480 | ArrayExpress
2012-08-19 | E-GEOD-31523 | ArrayExpress
2011-10-06 | E-GEOD-29196 | ArrayExpress
| GSE35379 | GEO
2012-02-27 | E-GEOD-35379 | ArrayExpress
2012-01-05 | E-GEOD-30047 | ArrayExpress
2020-01-23 | PXD006889 | Pride
2018-06-14 | E-MTAB-6956 | ArrayExpress