Transcriptomics

Dataset Information

6

Transcription profiling of human RKO cells after induction of KLF4 for different lengths of time


ABSTRACT: A stably transfected inducible system for KLF4 was established in RKO cells as previously described (Chen et al., 2001; Chen et al., 2003). This cell line, called EcR-RKO/KLF4, contains stably transfected receptors for the insect hormone, ecdysone (EcR), and a full-length mouse KLF4 cDNA driven by a promoter that responds to ecdysone and its analogue, ponasterone A. Cells were treated with 5 µM ponasterone A (PA), for various time durations. To control for the experiment, the vehicle, ethanol, was added for the same periods of time.

ORGANISM(S): Homo sapiens  

SUBMITTER: Vincent W. Yang  

PROVIDER: E-GEOD-3113 | ArrayExpress | 2007-06-21

SECONDARY ACCESSION(S): GDS1942GSE3113PRJNA93121

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptional profiling of the cell cycle checkpoint gene krüppel-like factor 4 reveals a global inhibitory function in macromolecular biosynthesis.

Whitney Erika M EM   Ghaleb Amr M AM   Chen Xinming X   Yang Vincent W VW  

Gene expression 20060101 2


Krüppel-like factor 4 (KLF4; also known as gut-enriched Krüppel-like factor or GKLF) is known to exhibit checkpoint function during the G1/S and G2/M transitions of the cell cycle. The mechanism by which KLF4 exerts these effects is not fully established. Here we investigated the expression profile of KLF4 in an inducible system over a time course of 24 h. Using oligonucleotide microarrays, we determined that the fold changes relative to control in expression levels of KLF4 exhibited a time-depe  ...[more]

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