Transcriptomics

Dataset Information

37

Transcription profiling by array of Arabidopsis knocked out for or over expressing mpk6


ABSTRACT: MPK6 shows transient increase in activity under hypoxia with maximal activity at 2 hrs. To study the role of MPK6 in hypoxia in Arabidopsis, 10 do seedlings of WT, mpk6 and MPK6 plants were exposed to 2 hrs hypoxia and 2hr air (mock). Because there are no major phenotypic differences between the genotypes, microarray was used to determine if there were transcript level differences between the genotypes. Wild type Col-0, MPK6 overexpression and mpk6 knockout mutants were exposed to 2 hr hypoxia and air and separated into root and shoot in order to determine the role of MPK6 in hypoxia in both root and shoot.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Julia Bailey-Serres  Ruth Chang   Cristina Branco-Price   Charles Jang   Peter Nghiem    

PROVIDER: E-GEOD-31158 | ArrayExpress | 2012-01-25

SECONDARY ACCESSION(S): GSE31158PRJNA144823

REPOSITORIES: GEO, ArrayExpress

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Publications

Transient MPK6 activation in response to oxygen deprivation and reoxygenation is mediated by mitochondria and aids seedling survival in Arabidopsis.

Chang Ruth R   Jang Charles J H CJ   Branco-Price Cristina C   Nghiem Peter P   Bailey-Serres Julia J  

Plant molecular biology 20111116 1-2


Mitogen-activated protein kinases (MPKs) are regulated by diverse stresses with a reactive oxygen species (ROS) component. Here, we report the rapid and transient activation of MPK3, MPK4 and MPK6 upon oxygen deprivation as well as reoxygenation in seedlings of Arabidopsis thaliana. MPK activation peaked within 2 h of oxygen deprivation and again at a higher magnitude within 5 min of reoxygenation. MPK6 was the predominant kinase regulated by oxygen availability in both aerial and root tissue, e  ...[more]

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