Transcriptomics

Dataset Information

3

Transcriptomic analysis of Bmi1 overexpression in NSPC.


ABSTRACT: We have previously shown that conditional overexpression of Bmi1 in NSC increases their proliferation both in the developing neocortexand in the postnatal brain (Yadirgi et al. 2011). However, during embryonic development, increased and ectopic proliferation induced by overexpression of Bmi1 in progenitors committed toward a neuronal lineage triggered apoptosis, leading eventually to a reduced overall brain size (Yadirgi et al. 2011). These findings –increased proliferation of neural stem/progenitor cells (NSPC) and apoptosis of neuronal committed progenitors - could be faithfully reproduced in an in vitro assay where NSPC isolated from Nestin-Cre; STOP FloxBmi1 embryos are cultured in floating conditions in the presence of EGF and FGF2 (neurosphere assay). We isolated NSPC from Nestin-Cre;STOP FloxBmi1 E16.5 neocortices and cultured them briefly under neurosphere inducing conditions. We then analysed their transcriptome by whole-genome Illumina platform mouse v2 and compared it to the transcriptome of NSPC isolated from non-transgenic or single transgenic littermates. The objective of this analysis was to identify genes differentially expressed upon overexpression of the PcG gene Bmi1 in neural stem/progenitor cells. 4 samples, 2 condition with 2 biological replicates per condition

ORGANISM(S): Mus musculus  

SUBMITTER: Danilo D Licastro   Silvia S Marino  Serena S Acquati  Danilo Licastro 

PROVIDER: E-GEOD-31607 | ArrayExpress | 2012-11-12

SECONDARY ACCESSION(S): GSE31607PRJNA145589

REPOSITORIES: GEO, ArrayExpress

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Publications

Epigenetic regulation of survivin by Bmi1 is cell type specific during corticogenesis and in gliomas.

Acquati Serena S   Greco Azzura A   Licastro Danilo D   Bhagat Heeta H   Ceric Dario D   Rossini Zefferino Z   Grieve Joan J   Shaked-Rabi Maya M   Henriquez Nick V NV   Brandner Sebastian S   Stupka Elia E   Marino Silvia S  

Stem cells (Dayton, Ohio) 20130101 1


Polycomb group proteins are essential regulators of stem cell function during embryonic development and in adult tissue homeostasis. Bmi1, a key component of the Polycomb Repressive Complex 1, is highly expressed in undifferentiated neural stem cells (NSC) as well as in several human cancers including high-grade gliomas--highly aggressive brain tumors. Using a conditional gene activation approach in mice, we show that overexpression of Bmi1 induces repressive epigenetic regulation of the promote  ...[more]

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