Dataset Information


Transcription profiling of mouse fibroblast cells infected with adenoviral vector or mock infected

ABSTRACT: The innate immune response to 1st generation adenoviral vectors was ascertained through high level infection of primary cells from a inbred mouse strain, replete with an intact and functioning TLR system. Experiment Overall Design: Quiescent murine C57/Bl6 fibroblasts were Ad (E1-E3-LacZ at MOI=300) or Mock infected. Sixteen hrs post-infection, RNA was harvested, transcribe to cDNA and bitotin-labeled, and hybridized to Mu11k A and B arrays.

ORGANISM(S): Mus musculus  

SUBMITTER: Zachary Conrad Hartman  

PROVIDER: E-GEOD-3172 | ArrayExpress | 2007-12-04



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Adenoviral infection induces a multi-faceted innate cellular immune response that is mediated by the toll-like receptor pathway in A549 cells.

Hartman Zachary C ZC   Black Esther P EP   Amalfitano Andrea A  

Virology 20061005 2

Adenovirus vectors are known to induce certain genes and impact innate response networks, but a broad understanding of this process and its mechanisms is currently lacking. For this reason, we chose to investigate and characterize Ad innate immunity using homogeneous, primary MEF cells replete with all the elements of the pathogen-sensing Toll-Like Receptor (TLR) pathway. By using an array-based approach to maximally define transcriptome changes induced upon Ad vector infection, we discovered th  ...[more]

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