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Omics score: 6

ATM Requirement in Gene Expression Responses to Ionizing Radiation in Human Lymphoblasts and Fibroblasts

ABSTRACT: Investigation of ATM-dependent and dose-dependent, or -independent, responses were examined in human lymphoblast cells 6 hr following exposure to either 1 or 5 Gy ionizing radiation. Human lymphoblast cells from "apparently healthy" individuals and individuals with Ataxia telangiectasia were exposed to 1 Gy or 5 Gy ionizing radiation. Gene expression responses 6 hr following IR were examined. Untreated samples were hybridized together with their matched treated samples.

OTHER RELATED OMICS DATASETS IN: PRJNA92717

ORGANISM(S): Homo sapiens

SUBMITTER: Cynthia L Innes William K Kaufmann Stella O Sieber Pierre R Bushel Alexandra N Heinloth Kristina G Flores Paula B Deming Richard S Paules Cynthia Innes

PROVIDER: E-GEOD-3174 | ArrayExpress | 2010-07-01

SECONDARY ACCESSION(S): GSE3174PRJNA92717

REPOSITORIES: GEO, ArrayExpress

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	E-GEOD-3174.README.txt	Txt
	E-GEOD-3174.idf.txt	Idf
	E-GEOD-3174.processed.1.zip	Processed
	E-GEOD-3174.sdrf.txt	Txt

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Publications

ATM requirement in gene expression responses to ionizing radiation in human lymphoblasts and fibroblasts.

Innes Cynthia L CL Heinloth Alexandra N AN Flores Kristina G KG Sieber Stella O SO Deming Paula B PB Bushel Pierre R PR Kaufmann William K WK Paules Richard S RS

Molecular cancer research : MCR 20060301 3

The heritable disorder ataxia telangiectasia (AT) is caused by mutations in the AT-mutated (ATM) gene with manifestations that include predisposition to lymphoproliferative cancers and hypersensitivity to ionizing radiation (IR). We investigated gene expression changes in response to IR in human lymphoblasts and fibroblasts from seven normal and seven AT-affected individuals. Both cell types displayed ATM-dependent gene expression changes after IR, with some responses shared and some responses v ...[more]

PMID: 16547157

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Project description:Humans are exposed to ionizing radiation (IR) from background radiation, medical treatments, occupational and accidental exposures. IR causes profound changes in transcription. Transcription is a primary process where protein amount and function can be regulated. One aspect of the transcriptional IR response that little is known about on a whole genome basis is alternative transcription. These investigations focus on the response to IR at the exon level in human cells but also at the whole gene level. Whole genome exon arrays were utilized to comprehensively characterize radiation-induced transcriptional expression products in two human cell types, namely EBV-transformed lymphoblast and primary fibroblast cell lines. 12 human primary fibroblast cell lines and 12 primary lymphoblast cell line samples were used for two doses (0 and 10 Gy) and two time points (0 and 4hr). Additional doses ( 1 Gy, 2 Gy, 5 Gy, and 20 Gy) and time points ( 1hr, 2hr, 8hr, 24hr and 48hr) were assessed in four lymphoblast cell lines and four primary fibroblasts.

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