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ATM Requirement in Gene Expression Responses to Ionizing Radiation in Human Lymphoblasts and Fibroblasts

ABSTRACT: Investigation of ATM-dependent and dose-dependent, or -independent, responses were examined in human lymphoblast cells 6 hr following exposure to either 1 or 5 Gy ionizing radiation. Human lymphoblast cells from "apparently healthy" individuals and individuals with Ataxia telangiectasia were exposed to 1 Gy or 5 Gy ionizing radiation. Gene expression responses 6 hr following IR were examined. Untreated samples were hybridized together with their matched treated samples.

ORGANISM(S): Homo sapiens

SUBMITTER: Cynthia L Innes William K Kaufmann Stella O Sieber Pierre R Bushel Alexandra N Heinloth Kristina G Flores Paula B Deming Richard S Paules Cynthia Innes

PROVIDER: E-GEOD-3174 | ArrayExpress | 2010-07-01

SECONDARY ACCESSION(S): GSE3174PRJNA92717

REPOSITORIES: GEO, ArrayExpress

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ATM requirement in gene expression responses to ionizing radiation in human lymphoblasts and fibroblasts.

Innes Cynthia L CL Heinloth Alexandra N AN Flores Kristina G KG Sieber Stella O SO Deming Paula B PB Bushel Pierre R PR Kaufmann William K WK Paules Richard S RS

Molecular cancer research : MCR 20060301 3

The heritable disorder ataxia telangiectasia (AT) is caused by mutations in the AT-mutated (ATM) gene with manifestations that include predisposition to lymphoproliferative cancers and hypersensitivity to ionizing radiation (IR). We investigated gene expression changes in response to IR in human lymphoblasts and fibroblasts from seven normal and seven AT-affected individuals. Both cell types displayed ATM-dependent gene expression changes after IR, with some responses shared and some responses v ...[more]

PMID: 16547157

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