Transcriptomics

Dataset Information

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Transcriptome-Wide Binding Sites for Components of the Saccharomyces cerevisiae Non-poly(A) termination Pathway: Nrd1, Nab3 and Sen1


ABSTRACT: We report here the transcriptome-wide distribution of yeast Rpb2, Sen1, Nrd1 and Nab3 binding sites. These data sets provide highresolution definition of non-poly(A) terminators, identify novel genes regulated by attenuation of nascent transcripts close to the promoter, and demonstrate the widespread occurrence of Nrd1-bound 3'-antisense transcripts on genes that are poorly expressed. In addition, we show that Sen1 does not cross-link to many expected ncRNAs but surprisingly binds to pre-mRNA transcripts suggesting a role in 3' end formation and/or termination. Six samples by adaptation of PAR-ClIP procedure

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Haiping Hao  Tyler Creamer   Jeff Corden   Paul Schaughency   Jeffry Corden   Nuttara Jamonnak   Sarah Wheelan   Miranda Darby    

PROVIDER: E-GEOD-31764 | ArrayExpress | 2011-09-08

SECONDARY ACCESSION(S): GSE31764SRP008118PRJNA145183

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing.

Jamonnak Nuttara N   Creamer Tyler J TJ   Darby Miranda M MM   Schaughency Paul P   Wheelan Sarah J SJ   Corden Jeffry L JL  

RNA (New York, N.Y.) 20110927 11


RNA polymerase II transcribes both coding and noncoding genes, and termination of these different classes of transcripts is facilitated by different sets of termination factors. Pre-mRNAs are terminated through a process that is coupled to the cleavage/polyadenylation machinery, and noncoding RNAs in the yeast Saccharomyces cerevisiae are terminated through a pathway directed by the RNA-binding proteins Nrd1, Nab3, and the RNA helicase Sen1. We have used an in vivo cross-linking approach to map  ...[more]

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