Transcriptomics

Dataset Information

4

Transcriptome profile in the human synovial MSC-aggregates


ABSTRACT: One of strategies to regenerate cartilage defect is transplantation of mesenchymal stem cells (MSCs). Improvements of therapeutic potential of MSCs are needed to achieve successful cartilage regeneration by transplantation of a limited number of cells. Aggregated culture is a popular method in ES and iPS cells to maintain or enhance their potentials. Here we investigated gene expression profile of aggregated MSCs. 621 genes were up-regulated and 409 genes were down-regulated more than 5-fold in MSC-aggregates compared with the number in MSCs in a monolayer culture. The most up-regulated gene was BMP2, which is one of the genes involved in chondrogenesis. Anti-inflammatory genes were also up-regulated in MSC-aggregates. The microarray data for selected genes were confirmed by real-time PCR. Human synovial MSCs was isolated from synovium of 3 distinct donors. The gene expression profile of MSC-aggregates cultured in hanging drop for 3days was compared with that of MSCs in a monolayer culture.

ORGANISM(S): Homo sapiens  

SUBMITTER: A Umezawa   H Makino  Akihiro Umezawa  K Tsuji  S Shiro  T Muneta  I Sekiya 

PROVIDER: E-GEOD-31980 | ArrayExpress | 2012-05-12

SECONDARY ACCESSION(S): GSE31980PRJNA144961

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2015-09-30 | E-MTAB-3795 | ArrayExpress
2015-12-19 | E-GEOD-76158 | ArrayExpress
2013-09-05 | E-GEOD-46989 | ArrayExpress
2013-01-28 | E-GEOD-43803 | ArrayExpress
2013-01-27 | E-GEOD-43781 | ArrayExpress
2015-04-30 | E-GEOD-54684 | ArrayExpress
2013-05-28 | E-GEOD-45499 | ArrayExpress
2015-05-07 | E-GEOD-59958 | ArrayExpress
2016-02-16 | PXD001952 | Pride
2014-11-18 | E-GEOD-29389 | ArrayExpress