Transcriptomics

Dataset Information

11

Gene Expression data from Mouse Balb/c Bone marrow derived macrophages infected by the promastigote form of Leishmania major parasite (P) or Killed parasite (Kp) during a time course of infection [Balb/c]


ABSTRACT: We analyzed the transcriptional signatures of mouse bone marrow-derived macrophages (BMDM) at different times after infection with promastigotes of the protozoan parasite Leishmania major. GeneChip Mouse Gene 1.0 ST arrays were used to analyse global changes in gene transcripts to generate a pool of genes that was statistically significant (p-value < 0.05) and a fold change cut-off of 1.5 in at least one of the five infected samples versus non-infected samples. Three independent biological replicates for each biological condition (Non infected "NI", infected with lived parasite "P" or "heat inactivated parasite "Kp") at 1h (T1), 3(T3), 6(T6), 12 (T12) and 24hours (T24) were performed. The Non infected (NI) at 0 hour (T0) biological condition were used as an additional control.

ORGANISM(S): Mus musculus  

SUBMITTER: Axel Rasche   Imen Rabhi  Béatrice Regnault  lamia Guizani-Tabbane  Andriani Daskalaki  Sameh Rabhi  Rym Ben-Othman  Lamia Guizani-Tabbane 

PROVIDER: E-GEOD-31995 | ArrayExpress | 2012-11-19

SECONDARY ACCESSION(S): GSE31995PRJNA155269

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptomic signature of Leishmania infected mice macrophages: a metabolic point of view.

Rabhi Imen I   Rabhi Sameh S   Ben-Othman Rym R   Rasche Axel A   Daskalaki Adriani A   Trentin Bernadette B   Piquemal David D   Regnault Béatrice B   Descoteaux Albert A   Guizani-Tabbane Lamia L  

PLoS neglected tropical diseases 20120821 8


We analyzed the transcriptional signatures of mouse bone marrow-derived macrophages at different times after infection with promastigotes of the protozoan parasite Leishmania major. Ingenuity Pathway Analysis revealed that the macrophage metabolic pathways including carbohydrate and lipid metabolisms were among the most altered pathways at later time points of infection. Indeed, L. major promastiogtes induced increased mRNA levels of the glucose transporter and almost all of the genes associated  ...[more]

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