Dataset Information


Pax6 mutant neocortex vs. wild type

ABSTRACT: Pax6 is a transcription factor with key functional roles in embryonic development. In order to identify downstream effectors of Pax6 in the developing cerebral cortex we performed microarray analysis. We compared gene expression profiles of cortical tissues isolated from wild type and Pax6-/- mouse embryos. In order to identify Pax6 downstream targets we carried out microarray analysis of Pax6-/- mutant mice. Pax6 is highly expressed in the mouse cerebral cortex at embryonic day E14.5, therefore we selected this tissue in order to compare gene expression profiles between wild type and Pax6-/- homozygous cortici. RNA samples were isolated from three mutant and three wild type embryos.

ORGANISM(S): Mus musculus  

SUBMITTER: V Tarabykin   S Boppana  R Geffers  Robert Geffers 

PROVIDER: E-GEOD-32271 | ArrayExpress | 2011-10-31



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Cellular retinaldehyde-binding protein (CRALBP) is a direct downstream target of transcription factor Pax6.

Boppana Sridhar S   Scheglov Alexander A   Geffers Robert R   Tarabykin Victor V  

Biochimica et biophysica acta 20111004 2

Transcription factor Pax6 plays an essential role in the expression of other transcription factors, cell adhesion molecules and is crucial for neurogenesis in the developing forebrain. Analysis of gene expression profiles through microarray experiments in Pax6 mutants allowed us to focus on CRALBP, one of the many genes that were downregulated.We studied the expression of CRALBP in wt and Pax6-/- mutants through in situ hybridization and immunohistochemistry. ChIP assay and luciferase reporter a  ...[more]

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