Transcriptomics

Dataset Information

3

How post-translational modification of nitrogenase is circumvented in Rhodopseudomonas palustris strains that produce hydrogen gas constitutively


ABSTRACT: Characterization of post-translational modification of nitrogenase in Rhodopseudomonas palustris strains that produce hydrogen gas constitutively. To characterize the effect of yeast extract as nitrogen source on gene expression under nitrogen fixing conditions, expression profiles of wild-type cells grown on either NF plus yeast extract and succinate and PM plus ammonium sulfate and succinate were compared using Affymetrix GeneChip. Using NF plus yeast extract and succinate media, transcriptome profiles of various mutants and wild-type cells were compared using glass spotted microarrays.

ORGANISM(S): Rhodopseudomonas palustris  

SUBMITTER: Yasuhiro Oda   Caroline S Harwood  Erin K Heiniger  Sudip K Samanta  Yasuhiro Oda 

PROVIDER: E-GEOD-32292 | ArrayExpress | 2012-03-15

SECONDARY ACCESSION(S): GSE32292PRJNA146363

REPOSITORIES: GEO, ArrayExpress

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How posttranslational modification of nitrogenase is circumvented in Rhodopseudomonas palustris strains that produce hydrogen gas constitutively.

Heiniger Erin K EK   Oda Yasuhiro Y   Samanta Sudip K SK   Harwood Caroline S CS  

Applied and environmental microbiology 20111216 4


Nitrogenase catalyzes the conversion of dinitrogen gas (N(2)) and protons to ammonia and hydrogen gas (H(2)). This is a catalytically difficult reaction that requires large amounts of ATP and reducing power. Thus, nitrogenase is not normally expressed or active in bacteria grown with a readily utilized nitrogen source like ammonium. nifA* mutants of the purple nonsulfur phototrophic bacterium Rhodopseudomonas palustris have been described that express nitrogenase genes constitutively and produce  ...[more]

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