Transcriptomics

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MiRNA expression profiles of trophoblastic cells


ABSTRACT: To gain more insight in the expression and role of microRNAs in trophoblastic cells, we have assessed the miRNA expression pattern of four trophoblastic cell lines and have compared them with the miRNA signature of human isolated 3rd trimester cytotrophoblast cells. Total RNA was reverse-transcribed, pre-amplified with miRNA-specific primers, and loaded into TLDA cards. Quantitative real-time PCR was performed for 768 miRNAs. For each of two cards per sample (A and B), 9 ul of pre-amplified diluted sample was mixed with TaqMan reaction mix (Applied Biosystems) and loaded into miRNA TLDA cards (Applied Biosystems) by centrifugation. Cards were sealed, and qRT-PCR was performed at a real-time thermocycler (ABI-7900, Applied Biosystems) following the manufacturer's recommendations. MiRNA signatures of human primary 3rd trimester trophoblast cells were compared with those of four trophoblastic cell lines

ORGANISM(S): Homo sapiens  

SUBMITTER: Udo R Markert  Udo R. Markert   Bernd Gruhn   Diana M Morales-Prieto   Wittaya Chaiwangyen    

PROVIDER: E-GEOD-32346 | ArrayExpress | 2011-09-22

SECONDARY ACCESSION(S): GSE32346PRJNA147243

REPOSITORIES: GEO, ArrayExpress

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