Transcriptomics

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Expression data from early Zebrafish embryos after knockdown of mir-34


ABSTRACT: microRNAs play crucial roles in the early development of an organism. However the regulation of transcription through the action of microRNAs during the initial embyonic development has not been studied. We used microarrays to detail the effect of maternal microRNA mir-34 in regulation of zygotic trancription during the initial stages of Zebrafish embryonic development from one cell stage through the maternal-zygotic transition up to 24 hours post fertilization. Zebrafish embryos were selected at specific stages of early embryonic development (1, 7, and 24 hours post fertilization). RNA was extracted and hybridized to Affymetrix microarrays. The embryos were injected with anti-microRNA LNA and were kept for constant monitoring for the indicated time points. A mockLNA was injected as a control. The embryos were visualized for any visible developmental abnormalities.

ORGANISM(S): Danio rerio  

SUBMITTER: Beena Pillai   Kartik Soni 

PROVIDER: E-GEOD-32360 | ArrayExpress | 2013-04-03

SECONDARY ACCESSION(S): GSE32360PRJNA147985

REPOSITORIES: GEO, ArrayExpress

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Publications

miR-34 is maternally inherited in Drosophila melanogaster and Danio rerio.

Soni Kartik K   Choudhary Ashwani A   Patowary Ashok A   Singh Angom Ramcharan AR   Bhatia Shipra S   Sivasubbu Sridhar S   Chandrasekaran Shanti S   Pillai Beena B  

Nucleic acids research 20130306 8


MicroRNAs (miRNAs) are small, endogenous, regulatory RNA molecules that can bind to partially complementary regions on target messenger RNAs and impede their expression or translation. We rationalized that miRNAs, being localized to the cytoplasm, will be maternally inherited during fertilization and may play a role in early development. Although Dicer is known to be essential for the transition from single-celled zygote to two-cell embryo, a direct role for miRNAs has not yet been demonstrated.  ...[more]

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