Genomics

Dataset Information

7

Autophagy driven by a master regulator of hematopoiesis


ABSTRACT: Developmental and homeostatic remodeling of cellular organelles is mediated by a complex process termed autophagy. The cohort of proteins that constitute the autophagy machinery function in a multistep biochemical pathway. Though components of the autophagy machinery are broadly expressed, autophagy can occur in specialized cellular contexts, and mechanisms underlying cell type-specific autophagy are poorly understood. We demonstrate that the master regulator of hematopoiesis GATA-1 directly activates transcription of genes encoding the essential autophagy component Microtubule Associated Protein 1 Light Chain 3B (LC3B) and its homologs (MAP1LC3A, GABARAP, GABARAPL1, GATE-16). In addition, GATA-1 directly activates genes involved in the biogenesis/function of lysosomes, which mediate autophagic protein turnover. We demonstrate that GATA-1 utilizes the forkhead protein FoxO3 to activate select autophagy genes. GATA-1-dependent LC3B induction is tightly coupled to accumulation of the active form of LC3B and autophagosomes, which mediate mitochondrial clearance as a critical step in erythropoiesis. These results illustrate a novel mechanism by which a master regulator of development establishes a genetic network to instigate cell type-specific autophagy. Genome-wide maps of GATA1 factor occupancy in primary human PBMC derived erythroblasts

ORGANISM(S): Homo Sapiens

SUBMITTER: Peggy J Farnham   Rajendran Sanalkumar  Chan-Jung Chang  Henriette O'Geen  Yoon-A Kang  Sunduz Keles  Emery H Bresnick  Eric E Bouhassira  Amelia K Linnemann 

PROVIDER: E-GEOD-32491 | ArrayExpress | 2011-09-30

SECONDARY ACCESSION(S): GSE32491SRP008518PRJNA147759

REPOSITORIES: GEO, ArrayExpress, ENA

Dataset's files

Source:
Action DRS
E-GEOD-32491.additional.1.zip Other
E-GEOD-32491.idf.txt Idf
E-GEOD-32491.processed.1.zip Processed
E-GEOD-32491.processed.2.zip Processed
E-GEOD-32491.processed.3.zip Processed
Items per page:
1 - 5 of 7
altmetric image

Publications


Developmental and homeostatic remodeling of cellular organelles is mediated by a complex process termed autophagy. The cohort of proteins that constitute the autophagy machinery functions in a multistep biochemical pathway. Though components of the autophagy machinery are broadly expressed, autophagy can occur in specialized cellular contexts, and mechanisms underlying cell-type-specific autophagy are poorly understood. We demonstrate that the master regulator of hematopoiesis, GATA-1, directly  ...[more]

Similar Datasets

2011-08-01 | E-GEOD-31092 | ArrayExpress
2018-06-14 | E-MTAB-6956 | ArrayExpress
2011-05-30 | E-GEOD-24849 | ArrayExpress
2008-10-18 | E-GEOD-7579 | ArrayExpress
2008-02-05 | E-GEOD-10134 | ArrayExpress
2011-10-06 | E-GEOD-26351 | ArrayExpress
| S-EPMC3255705 | BioStudies
| E-GEOD-32491 | BioStudies
2013-06-28 | E-GEOD-48332 | ArrayExpress
2013-02-05 | E-GEOD-44054 | ArrayExpress