Dataset Information


Gene Expression Profiles with Common Translocations in Acute Myeloid Leukemia (AML) using SAGE

ABSTRACT: SAGE was used to profile gene expression in leukemic myeloid progenitor cells of AML patients with t(8;21), t(15;17), t(9;11) and inv(16). Keywords: SAGE analysis of gene expression in leukemic myeloid progenitor cells isolated from bone marrow Bone marrow cells from 4 patients with de novo t(9;11), 3 with treatment-related t(9;11), and 5 each with inv(16), t(15;17), t(8;21) were used for the study. Each sample contained at least 75% affected cells with no other chromosome abnormalities at cytogenetic level. Mononuclear cells were purified from bone marrow cells for each sample, and CD15+ myeloid progenitor cells were isolated from these mononuclear cells. Poly A+ RNA were isolated from CD15+ myeloid progenitor cells of each sample for SAGE tag collection.

ORGANISM(S): Homo sapiens  

SUBMITTER: Theodore Karrison   Paul Liu  Masha Kocherginsky  San Ming Wang  Janet D Rowley (Correspondence)  Nimanthi Jayathilaka  Justin Kline  Jianjun Chen  Eric Green  Gerrard Bouffard  Yeong C Kim  Miao Sun  San M Wang (Correspondence)  Michelle LeBeau  Xijin Ge  Stefan Bohlander  Mark Minden  Richard Larson  Run Z Shi  Ozden Ozer  Neelmini Emmanuel  Jeffery Trent  Guolin Zhou  Sanggyu Lee 

PROVIDER: E-GEOD-3255 | ArrayExpress | 2005-11-30



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Identification of the specific cytogenetic abnormality is one of the critical steps for classification of acute myeloblastic leukemia (AML) which influences the selection of appropriate therapy and provides information about disease prognosis. However at present, the genetic complexity of AML is only partially understood. To obtain a comprehensive, unbiased, quantitative measure, we performed serial analysis of gene expression (SAGE) on CD15(+) myeloid progenitor cells from 22 AML patients who h  ...[more]

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