Transcriptomics

Dataset Information

20

Transcription profiling by array of human bone marrow hematopoietic stem cells from healthy young and elderly individuals


ABSTRACT: In the human hematopoietic system, aging is associated with decreased bone marrow cellularity, decreased adaptive immune system function, and increased incidence of anemia and other hematological disorders and malignancies. Recent studies in mice suggest that changes within the hematopoietic stem cell (HSC) population during aging contribute significantly to the manifestation of these age-associated hematopoietic pathologies. While the mouse HSC population has been shown to change both quantitatively and functionally with age, changes in the human HSC and progenitor cell populations during aging have not yet been characterized. Gene expression profiling revealed that aged human HSC transcriptionally up-regulate genes associated with cell cycle, myeloid lineage specification, and myeloid malignancies. These age-associated alterations in the frequency, function, and gene expression profile of human HSC are significantly similar to those changes observed in mouse HSC, suggesting that hematopoietic aging is an evolutionarily conserved process. In order to elucidate the properties of an aged human hematopoietic system that may predispose to age-associated hematopoietic dysfunction, we evaluated HSC and other hematopoietic progenitor populations from healthy, hematologically normal young and elderly human bone marrow samples. We found that aged human HSC increase in frequency, are less quiescent, and exhibit myeloid-biased differentiation potential compared to young HSC.

ORGANISM(S): Homo sapiens  

SUBMITTER: Elizabeth A Price   Isabel Beerman  Stanley L Schrier  Irving L Weissman  Derrick J Rossi  Debashis Sahoo  Wendy W Pang  William J Maloney 

PROVIDER: E-GEOD-32719 | ArrayExpress | 2011-11-11

SECONDARY ACCESSION(S): GSE32719PRJNA146789

REPOSITORIES: GEO, ArrayExpress

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Publications

Human bone marrow hematopoietic stem cells are increased in frequency and myeloid-biased with age.

Pang Wendy W WW   Price Elizabeth A EA   Sahoo Debashis D   Beerman Isabel I   Maloney William J WJ   Rossi Derrick J DJ   Schrier Stanley L SL   Weissman Irving L IL  

Proceedings of the National Academy of Sciences of the United States of America 20111128 50


In the human hematopoietic system, aging is associated with decreased bone marrow cellularity, decreased adaptive immune system function, and increased incidence of anemia and other hematological disorders and malignancies. Recent studies in mice suggest that changes within the hematopoietic stem cell (HSC) population during aging contribute significantly to the manifestation of these age-associated hematopoietic pathologies. Though the mouse HSC population has been shown to change both quantita  ...[more]

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