Transcriptomics

Dataset Information

3

HP1 wa191.S2.GFP_RNAi


ABSTRACT: modENCODE_submission_3707 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The goal of these experiments are to validate and confirm the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. To do this, we are using RNAi to deplete individual non-histone chromosomal proteins in Drosophila BG3 and S2 tissue culture cells, and then using antibodies to perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. Comparison of a protein factor's binding profiles before and after depletion will increase the confidence of our predictions. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: S2-DRSC; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Sex: Male; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line S2-DRSC; Antibody HP1 wa191 (target is HP1a); dsRNA (RNAi_reagent) Fly_GFP_RNAi&oldid=39872

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Nicole Riddle   Gary Karpen  DCC modENCODE  Vincenzo Pirrotta  Andrey Gortchakov  Art Alekseyenko  Greg Shanower  Michael Tolstorukov  Sarah Elgin  Aki Minoda  Mitzi Kuroda  Cameron Kennedy  Peter Park  Yuri Schwartz  Peter Kharchenko 

PROVIDER: E-GEOD-32802 | ArrayExpress | 2011-10-12

SECONDARY ACCESSION(S): GSE32802

REPOSITORIES: GEO, ArrayExpress

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