Transcriptomics

Dataset Information

303

SINGLET OXYGEN RESISTANT 1 links reactive electrophile signaling to singlet oxygen acclimation in Chlamydomonas reinhardtii


ABSTRACT: To identify components involved in the signal transduction and activation of the singlet oxygen-mediated response, a mutant selection was performed. This led to the isolation of the singlet oxygen resistant 1 (sor1) mutant, which is more tolerant to singlet oxygen-producing chemicals and shows a constitutively higher expression of GPXH and GSTS1. Map-based cloning revealed that the SOR1 gene encodes a novel bZIP transcription factor, which seems to control its own expression as well as that of a large number of oxidative stress response and detoxification genes. In the promoter region of many of these genes a highly conserved 8-bp palindromic sequence element was found to be enriched. This element was shown to be essential for GSTS1 overexpression in sor1 and for induction by increased levels of lipophilic reactive electrophile species (RES) suggesting that it functions as an electrophile response element (ERE). RES can be formed after singlet oxygen-induced lipid peroxidation, indicating that a RES-stimulated and SOR1-mediated response of detoxification genes is part of the singlet oxygen-induced acclimation process in C. reinhardtii. The sor1 mutant isolated in a screen for singlet oxygen resistant mutants and the corresponding wild-type strain 4A+ were grown in a 12 h light dark cycle for several days before total RNA was extracted 6 h after the light came on.

ORGANISM(S): Chlamydomonas reinhardtii  

SUBMITTER: Rik I Eggen   Sabeeha S Merchant  Beat B Fischer  David Casero  Heidi K Ledford  Setsuko Wakao  Andreas Koller  Krishna K Niyogi  Shih-Yau Huang  Matteo Pellegrini 

PROVIDER: E-GEOD-33548 | ArrayExpress | 2012-04-03

SECONDARY ACCESSION(S): SRP009273GSE33548PRJNA148881

REPOSITORIES: GEO, ArrayExpress, ENA

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SINGLET OXYGEN RESISTANT 1 links reactive electrophile signaling to singlet oxygen acclimation in Chlamydomonas reinhardtii.

Fischer Beat B BB   Ledford Heidi K HK   Wakao Setsuko S   Huang ShihYau Grace SG   Casero David D   Pellegrini Matteo M   Merchant Sabeeha S SS   Koller Andreas A   Eggen Rik I L RI   Niyogi Krishna K KK  

Proceedings of the National Academy of Sciences of the United States of America 20120423 20


Acclimation of Chlamydomonas reinhardtii cells to low levels of singlet oxygen, produced either by photoreactive chemicals or high light treatment, induces a specific genetic response that strongly increases the tolerance of the algae to subsequent exposure to normally lethal singlet oxygen-producing conditions. The genetic response includes the increased expression of various oxidative stress response and detoxification genes, like the glutathione peroxidase homologous gene GPXH/GPX5 and the σ-  ...[more]

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