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The expression profiles of extracellular matrix-related genes in the presence or absence of IL-17A or -17F as measured with the PCR array in systemic sclerosis (SSc) dermal fibroblasts

ABSTRACT: We examined the effect of IL-17 signaling pathway on extracellular matrix (ECM) expression and the involvement of IL-17 signaling pathway in pathogenesis of SSc. To identify differences in the expression pattern of ECM genes in IL-17A- or IL-17F-treated cells, we performed PCR array analysis, consisting of 84 ECM-related genes. Normal human dermal fibroblasts were cultured until they were confluent, and then stimulated with IL-17A or IL-17F for 12 hours, and total RNA was extracted. A mixture of equal amounts of mRNAs from three normal fibroblasts was prepared in the presence or absence of IL-17A or -17F, and mRNA expression profile was evaluated using PCR Array. Normal fibroblasts were obtained by skin biopsies from 3 healthy donors. Fibroblasts from donors were used and treated separately as indicated in the summary. Equal amount total RNA from each donor was pooled prior to gene expression analysis.

ORGANISM(S): Homo sapiens  

SUBMITTER: Taiji Naashima   Taiji Nakashima 

PROVIDER: E-GEOD-33581 | ArrayExpress | 2012-01-01



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Impaired IL-17 signaling pathway contributes to the increased collagen expression in scleroderma fibroblasts.

Nakashima Taiji T   Jinnin Masatoshi M   Yamane Keitaro K   Honda Noritoshi N   Kajihara Ikko I   Makino Takamitsu T   Masuguchi Shinichi S   Fukushima Satoshi S   Okamoto Yoshinobu Y   Hasegawa Minoru M   Fujimoto Manabu M   Ihn Hironobu H  

Journal of immunology (Baltimore, Md. : 1950) 20120307 8

Among IL-17 families, IL-17A and IL-17F share amino acid sequence similarity and bind to IL-17R type A. IL-17 signaling is implicated in the pathogenesis of various autoimmune diseases, but its role in the regulatory mechanism of extracellular matrix expression and its contribution to the phenotype of systemic sclerosis (SSc) both remain to be elucidated. This study revealed that IL-17A expression was significantly increased in the involved skin and sera of SSc patients, whereas the IL-17F level  ...[more]

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