Transcriptomics

Dataset Information

4

Gene expression in liver, gill, hypothalamus, pituitary, and olfactory rosettes from Atlantic salmon parr and smolt


ABSTRACT: To identify genes involved in the developmental process of Atlantic salmon smoltification, gene expression was compared between smolt and parr in tissues involved in osmoregulation (gill), metabolism (liver), imprinting (olfactory rosettes) and neuroendocrine control (hypothalamus and pituitary). Tissue samples were harvested from laboratory-reared parr and smolts on the same date. Smolts were distinguished from parr by size and appearance; developmental status was confirmed by physiological assays. Eight biological replicates (16 fish) balanced for sex and for dye were used in the liver, gill, olfactory rosette, and hypothalamus comparisons. Four male parr were compared to four male smolts and four female parr were compared to four female smolts; smolts were labeled with Alexa Fluor 555 on four arrays and with Alexa Fluor 647 on four arrays. Six biological replicates (12 fish) were used for the pituitary comparison (two female and four male).

ORGANISM(S): Salmo salar  

SUBMITTER: Laura S Robertson   Stephen D McCormick  Laura Robertson 

PROVIDER: E-GEOD-33711 | ArrayExpress | 2012-08-20

SECONDARY ACCESSION(S): GSE33711PRJNA148471

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Transcriptional profiling of the parr-smolt transformation in Atlantic salmon.

Robertson Laura S LS   McCormick Stephen D SD  

Comparative biochemistry and physiology. Part D, Genomics & proteomics 20120807 4


The parr-smolt transformation in Atlantic salmon (Salmo salar) is a complex developmental process that culminates in the ability to migrate to and live in seawater. We used GRASP 16K cDNA microarrays to identify genes that are differentially expressed in the liver, gill, hypothalamus, pituitary, and olfactory rosettes of smolts compared to parr. Smolts had higher levels of gill Na(+)/K(+)-ATPase activity, plasma cortisol and plasma thyroid hormones relative to parr. Across all five tissues, stri  ...[more]

Similar Datasets

2012-06-03 | E-GEOD-33715 | ArrayExpress
2020-01-01 | S-EPMC7544907 | BioStudies
2008-08-01 | E-TABM-449 | ArrayExpress
2010-06-01 | E-MEXP-2210 | ArrayExpress
2020-01-01 | S-EPMC6941806 | BioStudies
2017-01-01 | S-EPMC5292104 | BioStudies
2016-01-01 | S-EPMC5103829 | BioStudies
2013-04-18 | E-MTAB-1622 | ArrayExpress
1000-01-01 | S-EPMC4232760 | BioStudies
2014-06-20 | E-GEOD-58670 | ArrayExpress