Transcriptomics

Dataset Information

30

Effects of EVI1 mild expression in HeLa cells


ABSTRACT: We studied the variations of mRNA amounts after Flag-EVI1 or Flag expression in HeLa cells. Despites EVI1 discovery in 1988, its recognized role as a dominant oncogene in myeloid leukemia and more recently in epithelial cancers, only a few target genes were known and it was not clear why EVI1 was involved in cancer progression. Here we obtained the genomic binding occupancy and expression data for EVI1 in human cells. We identified numerous EVI1 target cancer genes and genes controlling cell migration and adhesion. Moreover, we characterized a transcriptional cooperation between AP1 and EVI1 that regulated proliferation and adhesion through a feed-forward loop. Furthermore, this study provides human genome-wide mapping and downstream analyses for EVI1 that will be useful for the research community. 8 samples were collected. Each condition was done in 4 replicates, collected 24 hours after transfection (for mild expression of EVI1). Transfections with Flag-expressing vector were used as controls.

ORGANISM(S): Homo sapiens  

SUBMITTER: Justin Jeyakani Joseph Gnanakkan  Neal G Copeland   Emilie A Bard-Chapeau   Guillaume Bourque   Justin Jeyakani    

PROVIDER: E-GEOD-33724 | ArrayExpress| 2012-01-23

SECONDARY ACCESSION(S): GSE33724PRJNA148447

REPOSITORIES: GEO, ArrayExpress

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