Transcriptomics,Multiomics

Dataset Information

12

TRNAs marked with CCACCA are targeted for degradation


ABSTRACT: The CCA-adding enzyme adds CCA to the 3' ends of transfer RNAs (tRNAs), a critical step in tRNA biogenesis that generates the amino acid attachment site. We found that the CCA-adding enzyme plays a key role in tRNA quality control by selectively marking unstable tRNAs and tRNA-like small RNAs for degradation. Instead of adding CCA to the 3' ends of these transcripts, CCA-adding enzymes from all three kingdoms of life add CCACCA. Here, we report deep sequencing analysis of the 3' ends of tRNA-Ser-CGA and tRNA-Ser-UGA from S. cerevisiae strains and show that hypomodified mature tRNAs are subjected to CCACCA (or poly(A) addition) as part of a rapid tRNA decay pathway in vivo. We conjecture that CCACCA addtion is a universal mechanism for controlling tRNA levels and preventing errors in translation. 121 samples analyzed in total, representing time courses of 10 different yeast strains; Biological replicates for each time point are included

OTHER RELATED OMICS DATASETS IN: PRJNA148403

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Joseph M Whipple   Eric M Phizicky  Jeremy E Wilusz  Phillip A Sharp 

PROVIDER: E-GEOD-33756 | ArrayExpress | 2011-11-17

SECONDARY ACCESSION(S): GSE33756SRP009362PRJNA148403

REPOSITORIES: GEO, ArrayExpress, ENA

Dataset's files

Source:
Action DRS
E-GEOD-33756.README.txt Txt
E-GEOD-33756.idf.txt Idf
E-GEOD-33756.processed.1.zip Processed
E-GEOD-33756.sdrf.txt Txt
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Publications

tRNAs marked with CCACCA are targeted for degradation.

Wilusz Jeremy E JE   Whipple Joseph M JM   Phizicky Eric M EM   Sharp Phillip A PA  

Science (New York, N.Y.) 20111101 6057


The CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase] adds CCA to the 3' ends of transfer RNAs (tRNAs), a critical step in tRNA biogenesis that generates the amino acid attachment site. We found that the CCA-adding enzyme plays a key role in tRNA quality control by selectively marking structurally unstable tRNAs and tRNA-like small RNAs for degradation. Instead of adding CCA to the 3' ends of these transcripts, CCA-adding enzymes from all three kingdoms of life add CCACCA. In addition, hy  ...[more]

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