Transcriptomics

Dataset Information

305

Genome-wide Transcriptional Responses of the Filamentous Fungus Aspergillus niger to Lignocellulose (Wheat straw) using RNA-sequencing


ABSTRACT: We have studied the physiological response of the fungus Aspergillus niger when exposed to wheat straw as a model lignocellulosic substrate. Using RNA-sequencing we showed that, 24 hours after exposure to straw, gene expression of known plant cell wall degrading enzymes represents a huge investment for the cells (about 20 % of the total mRNA). Our results also uncovered new esterases and surface interacting proteins that might form part of the fungal degradative arsenal. We also show that antisense transcripts are abundant and that their expression can be regulated by conditions. Triplicate samples of A. niger N402 taken at each of three timepoints: After 48 h growth in minimal media with Glucose as sole carbon source, After transfer to Wheat Straw media for 24 h and 5 h after after the exogenous addition of glucose to the wheat starw media.

ORGANISM(S): Aspergillus niger  

SUBMITTER: Gregory A Tucker   Roger Ibbet  Sunir Malla  Maria Campbell  Sanyasi Gaddipati  Matthew Kokolski  Aziz Aboobaker  Martin J Blythe  David Archer  Stéphane Delmas  Susan Liddell  David B Archer  Steven T Pullan 

PROVIDER: E-GEOD-33852 | ArrayExpress | 2012-08-27

SECONDARY ACCESSION(S): GSE33852SRP009412PRJNA148183

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Uncovering the genome-wide transcriptional responses of the filamentous fungus Aspergillus niger to lignocellulose using RNA sequencing.

Delmas Stéphane S   Pullan Steven T ST   Gaddipati Sanyasi S   Kokolski Matthew M   Malla Sunir S   Blythe Martin J MJ   Ibbett Roger R   Campbell Maria M   Liddell Susan S   Aboobaker Aziz A   Tucker Gregory A GA   Archer David B DB  

PLoS genetics 20120809 8


A key challenge in the production of second generation biofuels is the conversion of lignocellulosic substrates into fermentable sugars. Enzymes, particularly those from fungi, are a central part of this process, and many have been isolated and characterised. However, relatively little is known of how fungi respond to lignocellulose and produce the enzymes necessary for dis-assembly of plant biomass. We studied the physiological response of the fungus Aspergillus niger when exposed to wheat stra  ...[more]

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