Dataset Information


Transcription profiling by array of mouse thymic medullary epithelial cells mutant for Aire

ABSTRACT: The aim of this study is to analyze the transcriptional effects of Aire deficiency in the thymus, using the Affymetrix MoGene platform to analyze variation in exon usage MECs were isolated from 4-6 wk-old WT or Aire KO ((B6xNOD)F1 background) mice. Three WT and three Aire-KO mice taken individually were used.


ORGANISM(S): Mus musculus  

SUBMITTER: Matthieu Giraud   Christophe Benoist  CBDM Z. Lab 

PROVIDER: E-GEOD-33878 | ArrayExpress | 2011-11-23



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Aire unleashes stalled RNA polymerase to induce ectopic gene expression in thymic epithelial cells.

Giraud Matthieu M   Yoshida Hideyuki H   Abramson Jakub J   Rahl Peter B PB   Young Richard A RA   Mathis Diane D   Benoist Christophe C  

Proceedings of the National Academy of Sciences of the United States of America 20111227 2

Aire is a transcriptional regulator that induces expression of peripheral tissue antigens (PTA) in thymic medullary epithelial cells (MECs), driving immunological self-tolerance in differentiating T cells. To elucidate its mechanistic pathways, we examined its transcriptional impact in MECs in vivo by microarray analysis with mRNA-spanning probes. This analysis revealed initiation of Aire-activated genes to be comparable in Aire-deficient and wild-type MECs, but with a block to elongation after  ...[more]

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