Transcriptomics

Dataset Information

240

Retrotransposon Ty1 integration targets specifically positioned asymmetric nucleosomal DNA segments in tRNA hotspots


ABSTRACT: Using a TIP-seq protocol (specifically isolating transposon insertion junctions) we determined that the Ty1 retrotransposon targets tRNA genes and, in particular, we determined that the transposon inserts into nucleosomal DNA in an asymmetric pattern. TIP-seq recovery of transposon insertion junctions in haploid and diploid yeast

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Sarah J Wheelan   Jef D Boeke 

PROVIDER: E-GEOD-33986 | ArrayExpress | 2012-01-01

SECONDARY ACCESSION(S): SRP009462GSE33986PRJNA150613

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Retrotransposon Ty1 integration targets specifically positioned asymmetric nucleosomal DNA segments in tRNA hotspots.

Mularoni Loris L   Zhou Yulian Y   Bowen Tyson T   Gangadharan Sunil S   Wheelan Sarah J SJ   Boeke Jef D JD  

Genome research 20120104 4


The Saccharomyces cerevisiae genome contains about 35 copies of dispersed retrotransposons called Ty1 elements. Ty1 elements target regions upstream of tRNA genes and other Pol III-transcribed genes when retrotransposing to new sites. We used deep sequencing of Ty1-flanking sequence amplicons to characterize Ty1 integration. Surprisingly, some insertions were found in mitochondrial DNA sequences, presumably reflecting insertion into mitochondrial DNA segments that had migrated to the nucleus. Th  ...[more]

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