Transcriptomics

Dataset Information

6

Expression data of Sox9+ and Ngn3+ mouse pancreas cells at different stages of development


ABSTRACT: Genes specific to Sox9+ pancreatic progenitors were identified by comparing the gene expression in embryonic and adult Sox9+ cells. We used microarray analysis to detail the global changes in gene expression as Sox9 positive embryonic pancreatic progenitors differentiatiate into adult ductal cells or the endocrine lineage. GFP positive cells from Sox9-EGFP mouse pancreas were isolated by FACS at different stages of development (e10.5, e15.5, and p23) for RNA extraction and hybridization to Affymetrix microarrays. To obtain populations highly enriched in Sox9 expression, we collected only GFP Hi populations for analysis. To identify gene expression changes specific to the differentiation of progenitors to ductal cells or endocrine cells, we also isolated and analyzed the gene expression profile of GFP negative cells isolated at p23, as well as GFP positive cells isolated from Ngn3-EGFP mouse pancreas at e15.5. These two populations allow the identification of genes whose expression is associated with the newly differentiated endocrine progeny in the embryo (Ngn3-GFP positive) and adult acinar and endocrine cells at p23.

ORGANISM(S): Mus musculus  

SUBMITTER: Kristine Freude   Janel L Kopp  Maike Sander  Hung Ping Shih 

PROVIDER: E-GEOD-34060 | ArrayExpress | 2012-08-01

SECONDARY ACCESSION(S): GSE34060PRJNA150017

REPOSITORIES: GEO, ArrayExpress

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Publications

A Notch-dependent molecular circuitry initiates pancreatic endocrine and ductal cell differentiation.

Shih Hung Ping HP   Kopp Janel L JL   Sandhu Manbir M   Dubois Claire L CL   Seymour Philip A PA   Grapin-Botton Anne A   Sander Maike M  

Development (Cambridge, England) 20120606 14


In the pancreas, Notch signaling is thought to prevent cell differentiation, thereby maintaining progenitors in an undifferentiated state. Here, we show that Notch renders progenitors competent to differentiate into ductal and endocrine cells by inducing activators of cell differentiation. Notch signaling promotes the expression of Sox9, which cell-autonomously activates the pro-endocrine gene Ngn3. However, at high Notch activity endocrine differentiation is blocked, as Notch also induces expre  ...[more]

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