Transcriptomics

Dataset Information

5

Proinflammatory role of histamine-releasing factor in asthma and allergy


ABSTRACT: Immunoglobulin (Ig) E-mediated activation of mast cells and basophils underlies allergic diseases such as asthma. Histamine-releasing factor (HRF), also known as translationally controlled tumor protein (TCTP) and fortilin, is a highly conserved protein with both intracellular and extracellular functions. Secreted HRF can stimulate histamine release and IL-4 and IL-13 production from IgE-sensitized basophils and mast cells. HRF is found in nasal, skin blister and bronchoalveolar lavage (BAL) fluids during late-phase allergic reactions (LPRs), which implicates HRF in the LPR and chronic allergic inflammation. Here we identify a subset of IgE and IgG antibodies as HRF-interacting molecules. HRF can exist as a dimer and bind to immunoglobulins (Igs) via interactions of its N-terminal and internal regions with the Fab region of Igs. Therefore, HRF together with HRF-reactive IgE can activate mast cells in vitro. The Ig-interacting HRF peptides that block HRF-Ig interactions can inhibit IgE+HRF-induced mast cell activation and in vivo cutaneous anaphylaxis and airway inflammation. Intranasally administered HRF can recruit inflammatory immune cells to the lung in naïve mice in a mast cell- and Fc receptor-dependent manner. These results strongly suggest the proinflammatory role of HRF in asthma and skin immediate hypersensitivity. A total of 6 samples were analyzed; wild type C57BL/6, FcRg KO and FceRIa KO mice were challenged with PBS (control) or mouse histamien-releasing factor

ORGANISM(S): Mus musculus  

SUBMITTER: Miho Kimura   Jun-ichi Kashiwakura  Kenji Matsumoto  Jiro Kitaura  Reuben P Siraganian  Toshiaki Kawakami  Susan M MacDonald  Michael H Matho  Chisei Ra  Tomoaki Ando  Dirk M Zajonc  Yuko Kawakami  David H Broide  Tomomitsu Ozeki 

PROVIDER: E-GEOD-34133 | ArrayExpress | 2011-12-04

SECONDARY ACCESSION(S): GSE34133PRJNA149851

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2005-09-09 | GSE3271 | GEO
| GSE96696 | GEO
2018-07-04 | PXD010047 | Pride
| GSE96694 | GEO
| GSE96695 | GEO
2012-05-02 | GSE35332 | GEO
2012-05-01 | E-GEOD-35332 | ArrayExpress
2014-07-03 | E-GEOD-35332 | ExpressionAtlas
2013-04-01 | E-GEOD-41596 | ArrayExpress
2009-03-12 | E-GEOD-15174 | ArrayExpress