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Dietary heme modulates microbiota and mucosa of mouse colon without significant host-microbe cross talk

ABSTRACT: Previously, we showed that dietary heme injured the colonic surface epithelium and induced hyperproliferation by changing the surface to crypt signaling. In this study we investigated whether bacteria play a role in this changed signaling. Dietary heme increased the Bacteroidetes and decreased the Firmicutes in colonic content. This shift was caused by a selective susceptibility of Gram-positive bacteria to the heme cytotoxic fecal waters, which is not observed for Gram-negative bacteria allowing expansion of the Gram-negative community. The increased amount of Gram-negative bacteria increased LPS exposure to colonocytes, however, there is no appreciable immune response detected in the heme-fed mice. There were no signs of sensing of the bacteria by the mucosa, as changes in TLR signaling were not present. This lack of microbe-host cross talk indicated that the changes in microbiota do not play a causal role in the heme-induced hyperproliferation. Mice received control or heme diet for 14 days, whereafter pooled colon samples were analysed on microarrays.

ORGANISM(S): Mus musculus  

SUBMITTER: Anneke Rijnierse   Noortje IJssennagger  Roelof v Meer  Guido Hooiveld  Michael Muller 

PROVIDER: E-GEOD-34253 | ArrayExpress | 2013-07-18



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Dietary heme alters microbiota and mucosa of mouse colon without functional changes in host-microbe cross-talk.

IJssennagger Noortje N   Derrien Muriel M   van Doorn Gerdien M GM   Rijnierse Anneke A   van den Bogert Bartholomeus B   Müller Michael M   Dekker Jan J   Kleerebezem Michiel M   van der Meer Roelof R  

PloS one 20121211 12

Colon cancer is a major cause of cancer deaths in Western countries and is associated with diets high in red meat. Heme, the iron-porphyrin pigment of red meat, induces cytotoxicity of gut contents which injures surface cells leading to compensatory hyperproliferation of crypt cells. This hyperproliferation results in epithelial hyperplasia which increases the risk of colon cancer. In humans, a high red-meat diet increases Bacteroides spp in feces. Therefore, we simultaneously investigated the e  ...[more]

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