Dataset Information


Expression of Nodule Cysteine-Rich (NCR) Defensin-Like (DEFL) genes among four different accessions of Medicago truncatula inoculated with two different rhizobial strains.

ABSTRACT: We have undertaken a detailed study to identify expression polymorphism of NCRs. We used a custom Affymetrix oligonucleotide microarray to examine the expression changes of 566 NCRs in four different accessions of Medicago truncatula. Each accession was inoculated with two different rhizobial strains to assess the NCR expression differences caused by host-symbiont specificity. The NCRs displayed significant expression differences among the ecotypes but had subtle differences in gene expression because of strain difference. We used a custom Affymetrix chip containing 684 probe sequences of Medicago DEFLs to explore the expression polymorphism of NCRs in nodules inoculated with Sinorhizobium meliloti 1021(Sm1021) and Sinorhizobium medicae (SmA321) totalling 8 different treatments. Each treatment was supported by three biological replicates giving a grand total of 24 samples.

ORGANISM(S): Medicago truncatula  

SUBMITTER: Peng Zhou   Kevin A Silverstein  Nevin D Young  Sumitha Nallu  Kathryn A VandenBosch  Peter Tiffin 

PROVIDER: E-GEOD-34804 | ArrayExpress | 2014-06-20



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Patterns of divergence of a large family of nodule cysteine-rich peptides in accessions of Medicago truncatula.

Nallu Sumitha S   Silverstein Kevin A T KA   Zhou Peng P   Young Nevin D ND   Vandenbosch Kathryn A KA  

The Plant journal : for cell and molecular biology 20140423 4

The nodule cysteine-rich (NCR) groups of defensin-like (DEFL) genes are one of the largest gene families expressed in the nodules of some legume plants. They have only been observed in the inverted repeat loss clade (IRLC) of legumes, which includes the model legume Medicago truncatula. NCRs are reported to play an important role in plant-microbe interactions. To understand their diversity we analyzed their expression and sequence polymorphisms among four accessions of M. truncatula. A significa  ...[more]

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