Transcriptomics

Dataset Information

7

Development of miRNA expression signatures of MEFs deficient in a ER stress mediator XBP1 for tunicamycin treatment


ABSTRACT: To further development of our miRNA expression approach to ER stress, we have employed miRNA microarray expression profiling as a discovery platform to identify ER stress-responsible ones. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator XBP1 were treated with tunicamycin for 12 or 24 hrs. miRNAs responsible for tunicamycin-treatment for 12hrs in XBP1-dependent manner were extracted. Among them, expression of three miRNAs (miR-23a, miR-27a, miR-24-2) was quantified in the RNA samples from the same as the microarray, and COS7 cells by real-time PCR, confirming existence of similar mechanisms of trancriptional repression in ER stress by tunicamycin treatment. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator XBP1 were treated with 2ug/mL tunicamycin for 12 or 24 hrs. Two independent experiments were performed at each time (untreated, 12 or 24 hrs). miRNAs responsible for tunicamycin-treatment for 12hrs in XBP1-dependent manner were extracted.

ORGANISM(S): Mus musculus  

SUBMITTER: Naoko Miura   Kazue Tsugawa  Miho Oyadomari  Mari Funahashi  Tomoko Mori  Kazuna Takahara  Taiji Ito  Robert Zheng  Kiyoe Kurahashi  Ryosuke Sato  Chinobu Miyamoto  Masato Miyake  Seiichi Oyadomari 

PROVIDER: E-GEOD-35174 | ArrayExpress | 2015-01-18

SECONDARY ACCESSION(S): GSE35174PRJNA155983

REPOSITORIES: GEO, ArrayExpress

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